Abstract：
Bumblebees are crucial pollinators, providing essential ecosystem services and global food production. The success of pollination services relies on the interaction between sensory organs and the environment. The antenna functions as a versatile multi-sensory organ, pivotal in mediating chemosensory/olfactory information, and governs adaptive responses to environmental changes. Despite an increasing number of RNA-sequencing studies on insect antenna, comprehensive antennal transcriptome studies at the different life stages were not elucidated systematically. Here, we quantified the expression profile and dynamics of coding/microRNA genes of larval head and antennal tissues from early- and late-stage pupa to the adult of Bombus terrestris as suitable model organism among pollinators. We further performed Pearson correlation analyses on the gene expression profiles of the antennal transcriptome from larval head tissue to adult stages, exploring both positive and negative expression trends. The positively correlated coding genes were primarily enriched in sensory perception of chemical stimuli, ion transport, transmembrane transport processes and olfactory receptor activity. Negatively correlated genes were mainly enriched in organic substance biosynthesis and regulatory mechanisms underlying larval body patterning and the formation of juvenile antennal structures. As post-transcriptional regulators, miR-1000-5p, miR-13b-3p, miR-263-5p and miR-252-5p showed positive correlations, whereas miR-315-5p, miR-92b-3p, miR-137-3p, miR-11-3p and miR-10-3p exhibited negative correlations in antennal tissue. Notably, based on the inverse expression relationship, positively and negatively correlated microRNA (miRNA)-mRNA target pairs revealed that differentially expressed miRNAs predictively targeted genes involved in antennal development, shaping antennal structures and regulating antenna-specific functions. Our data serve as a foundation for understanding stage-specific antennal transcriptomes and large-scale comparative analysis of transcriptomes in different insects.
摘要：
大黄蜂是至关重要的授粉者,提供基本的生态系统服务和全球粮食生产。授粉服务的成功依赖于感觉器官与环境之间的相互作用。天线作为一个多功能的多感觉器官，在调节化学感觉/嗅觉信息方面至关重要，并控制对环境变化的适应性反应。尽管对昆虫天线的RNA测序研究越来越多，不同生命阶段的全面触角转录组研究尚未系统阐明。这里,我们量化了从早期和晚期the到Bombusterrais成虫的幼虫头和触角组织的编码/microRNA基因的表达谱和动力学，作为传粉者中合适的模型生物。我们进一步对从幼虫头部组织到成虫阶段的触角转录组的基因表达谱进行了Pearson相关性分析，探索积极和消极的表达趋势。正相关的编码基因主要富集在化学刺激的感官知觉中，离子传输，跨膜转运过程和嗅觉受体活性。负相关的基因主要集中在有机物质的生物合成以及幼体模式和幼体触角结构形成的调节机制中。作为转录后调节因子，miR-1000-5p,miR-13b-3p,miR-263-5p和miR-252-5p呈正相关,而miR-315-5p，miR-92b-3p,miR-137-3p,miR-11-3p和miR-10-3p在触角组织中呈负相关。值得注意的是,基于逆表达式关系，正相关和负相关的microRNA（miRNA）-mRNA靶对显示差异表达的miRNA预测靶向参与触角发育的基因，塑造触角结构和调节天线特定功能。我们的数据为了解特定阶段的触角转录组以及对不同昆虫的转录组进行大规模比较分析奠定了基础。
