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Abstract：
Lumpy skin disease virus (LSDV) is a member of the capripoxvirus (CPPV) genus of the Poxviridae family. LSDV is a rapidly emerging, high-consequence pathogen of cattle, recently spreading from Africa and the Middle East into Europe and Asia. We have sequenced the whole genome of historical LSDV isolates from the Pirbright Institute virus archive, and field isolates from recent disease outbreaks in Sri Lanka, Mongolia, Nigeria and Ethiopia. These genome sequences were compared to published genomes and classified into different subgroups. Two subgroups contained vaccine or vaccine-like samples (\"Neethling-like\" clade 1.1 and \"Kenya-like\" subgroup, clade 1.2.2). One subgroup was associated with outbreaks of LSD in the Middle East/Europe (clade 1.2.1) and a previously unreported subgroup originated from cases of LSD in west and central Africa (clade 1.2.3). Isolates were also identified that contained a mix of genes from both wildtype and vaccine samples (vaccine-like recombinants, grouped in clade 2). Whole genome sequencing and analysis of LSDV strains isolated from different regions of Africa, Europe and Asia have provided new knowledge of the drivers of LSDV emergence, and will inform future disease control strategies.
摘要：
块状皮肤病病毒（LSDV）是Poxviridae家族的羊痘病毒（CPPV）属的成员。LSDV是一种迅速崛起的,牛的高后果病原体，最近从非洲和中东蔓延到欧洲和亚洲。我们已经对Pirbright研究所病毒档案中的历史LSDV分离株的全基因组进行了测序，以及斯里兰卡最近爆发的疾病的现场分离株，蒙古,尼日利亚和埃塞俄比亚。将这些基因组序列与已发表的基因组进行比较，并分为不同的亚组。两个亚组包含疫苗或疫苗样样本(“Neethling样”进化枝1.1和“Kenya样”亚组，进化枝1.2.2)。一个亚组与中东/欧洲的LSD暴发有关（进化枝1.2.1），一个以前未报告的亚组起源于西非和中非的LSD病例（进化枝1.2.3）。还鉴定了包含来自野生型和疫苗样品（疫苗样重组体，分组为进化枝2)。从非洲不同地区分离的LSDV菌株的全基因组测序和分析，欧洲和亚洲提供了有关LSDV出现的驱动因素的新知识，并将为未来的疾病控制策略提供信息。
