PDF(Pubmed)
Abstract：
PRRS is a viral disease that profoundly impacts the global swine industry, causing significant economic losses. The development of a novel and effective vaccine is crucial to halt the rapid transmission of this virus. There have been several vaccination attempts against PRRSV using both traditional and alternative vaccine design development approaches. Unfortunately, there is no currently available vaccine that can completely control this disease. Thus, our study aimed to develop an mRNA vaccine using the antigens expressed by single or fused PRRSV structural proteins. In this study, the nucleotide sequence of the immunogenic mRNA was determined by considering the antigenicity of structural proteins and the stability of spatial structure. Purified GP5 protein served as the detection antigen in the immunological evaluation. Furthermore, cellular mRNA expression was detected by immunofluorescence and western blotting. In a mice experiment, the Ab titer in serum and the activation of spleen lymphocytes triggered by the antigen were detected by ELISA and ICS, respectively. Our findings demonstrated that both mRNA vaccines can significantly stimulate cellular and humoral immune responses. More specifically, the GP5-mRNA exhibited an immunological response that was similar to that of the commercially available vaccine when administered in high doses. To conclude, our vaccine may show promising results against the wild-type virus in a natural host.
摘要：
PRRS是一种病毒性疾病，对全球养猪业产生深远的影响。造成重大经济损失。新型有效疫苗的开发对于阻止该病毒的快速传播至关重要。已经有几种使用传统和替代疫苗设计开发方法的针对PRRSV的疫苗接种尝试。不幸的是,目前没有可用的疫苗可以完全控制这种疾病。因此，我们的研究旨在利用单一或融合PRRSV结构蛋白表达的抗原开发一种mRNA疫苗.在这项研究中,通过考虑结构蛋白的抗原性和空间结构的稳定性来确定免疫原性mRNA的核苷酸序列。纯化的GP5蛋白在免疫学评价中用作检测抗原。此外，通过免疫荧光和蛋白质印迹法检测细胞mRNA的表达。在老鼠实验中，用ELISA和ICS检测血清Ab滴度和抗原触发的脾淋巴细胞活化,分别。我们的发现表明，两种mRNA疫苗都可以显着刺激细胞和体液免疫反应。更具体地说,GP5-mRNA在高剂量给药时表现出与市售疫苗相似的免疫应答.最后，我们的疫苗可能在天然宿主中对野生型病毒显示出有希望的结果.
