PDF(Pubmed)
Abstract：
Ticks are the main arthropod vector of pathogens to humans and livestock in the British Isles. Despite their role as a vector of disease, many aspects of tick biology, ecology, and microbial association are poorly understood. To address this, we investigated the composition of the microbiome of adult and nymphal Ixodes ricinus ticks. The ticks were collected on a dairy farm in Southwest England and RNA extracted for whole genome sequencing. Sequences were detected from a range of microorganisms, particularly tick-associated viruses, bacteria, and nematodes. A majority of the viruses were attributed to phlebo-like and nairo-like virus groups, demonstrating a high degree of homology with the sequences present in I. ricinus from mainland Europe. A virus sharing a high sequence identity with Chimay rhabdovirus, previously identified in ticks from Belgium, was detected. Further investigations of I. ricinus ticks collected from additional sites in England and Wales also identified Chimay rhabdovirus viral RNA with varying prevalence in all tick populations. This suggests that Chimay rhabdovirus has a wide distribution and highlights the need for an extended exploration of the tick microbiome in the United Kingdom (UK).
摘要：
蜱是不列颠群岛病原体对人类和牲畜的主要节肢动物媒介。尽管它们是疾病的媒介，蜱生物学的许多方面，生态学,和微生物的关联知之甚少。为了解决这个问题，我们调查了成年和若虫蓖麻蜱的微生物组组成。在英格兰西南部的一个奶牛场收集蜱，并提取RNA进行全基因组测序。从一系列微生物中检测到序列，特别是与蜱相关的病毒，细菌,和线虫。大多数病毒都归于类和类病毒组，与来自欧洲大陆的I.ricinus中存在的序列具有高度同源性。一种与黑猩猩弹状病毒具有高度序列同一性的病毒，以前在比利时的蜱中发现，被检测到。对从英格兰和威尔士其他地点收集的I.ricinustick的进一步调查还确定了在所有tick种群中具有不同患病率的Chimay弹状病毒病毒RNA。这表明Chimay弹状病毒具有广泛的分布，并强调了在英国（UK）对tick微生物组进行扩展探索的必要性。
