PDF(Pubmed)
Abstract：
BACKGROUND: Although the adaptive immune responses to the CoronaVac vaccine are known, their dynamics in indigenous communities remain unclear. In this study, we assessed the humoral and cellular immune responses to CoronaVac (Sinovac Biotech Life Sciences, 2021 NCT05225285, Beijing, China), in immunized Brazilian indigenous individuals.
METHODS: We conducted a prospective cohort study on indigenous Brazilian people between February 2021 and June 2021. Analyses of immune responses were carried out before (T1) and after a vaccination schedule was completed (T2). Demographic data were collected using a questionnaire.
RESULTS: We initially included 328 patients; among them, 120 (36.6%) had no SARS-CoV-2 antibodies. Peripheral blood mononuclear cells (PBMCs) were collected from 106 patients during follow-up visits, of which 91 samples were analyzed by immunophenotyping assay to detect SARS-CoV-2-specific memory T-cell response. Post-vaccination, the levels of memory B-cells and Natural Killer T-lymphocytes increased. Bororó village residents, females, and Terena ethnic group members had higher levels of anti-spike IgG antibodies post-vaccination, whereas alcohol and tobacco users had lower concentrations.
CONCLUSIONS: To our best knowledge, this was the first comprehensive assessment of antibody and T-cell responses against CoronaVac vaccination in indigenous patients. Our findings showed that antibody response and T-cell immunity against SARS-CoV-2 were present in most patients following the vaccination schedule.
METHODS: We conducted a prospective cohort study on indigenous Brazilian people between February 2021 and June 2021. Analyses of immune responses were carried out before (T1) and after a vaccination schedule was completed (T2). Demographic data were collected using a questionnaire.
RESULTS: We initially included 328 patients; among them, 120 (36.6%) had no SARS-CoV-2 antibodies. Peripheral blood mononuclear cells (PBMCs) were collected from 106 patients during follow-up visits, of which 91 samples were analyzed by immunophenotyping assay to detect SARS-CoV-2-specific memory T-cell response. Post-vaccination, the levels of memory B-cells and Natural Killer T-lymphocytes increased. Bororó village residents, females, and Terena ethnic group members had higher levels of anti-spike IgG antibodies post-vaccination, whereas alcohol and tobacco users had lower concentrations.
CONCLUSIONS: To our best knowledge, this was the first comprehensive assessment of antibody and T-cell responses against CoronaVac vaccination in indigenous patients. Our findings showed that antibody response and T-cell immunity against SARS-CoV-2 were present in most patients following the vaccination schedule.
摘要：
背景：尽管对CoronaVac疫苗的适应性免疫反应是已知的，他们在土著社区的动态仍不清楚。在这项研究中,我们评估了对CoronaVac（Sinovac生物技术生命科学，2021NCT05225285,北京,中国),接种疫苗的巴西土著人。
方法：我们在2021年2月至2021年6月期间对巴西土著人民进行了一项前瞻性队列研究。在完成疫苗接种时间表之前(T1)和之后(T2)进行免疫应答的分析。使用问卷收集人口统计学数据。
结果：我们最初纳入了328名患者；其中，120例(36.6%)没有SARS-CoV-2抗体。在随访期间收集106名患者的外周血单核细胞（PBMC），其中91个样本通过免疫表型分析来检测SARS-CoV-2特异性记忆T细胞反应。接种疫苗后，记忆B细胞和自然杀伤T淋巴细胞的水平增加。博罗罗村居民，女性,和Terena族成员在接种疫苗后有更高水平的抗尖峰IgG抗体，而酒精和烟草使用者的浓度较低。
结论：据我们所知，这是首次全面评估土著患者针对CoronaVac疫苗接种的抗体和T细胞反应.我们的发现表明，按照疫苗接种时间表，大多数患者都存在针对SARS-CoV-2的抗体反应和T细胞免疫。
方法：我们在2021年2月至2021年6月期间对巴西土著人民进行了一项前瞻性队列研究。在完成疫苗接种时间表之前(T1)和之后(T2)进行免疫应答的分析。使用问卷收集人口统计学数据。
结果：我们最初纳入了328名患者；其中，120例(36.6%)没有SARS-CoV-2抗体。在随访期间收集106名患者的外周血单核细胞（PBMC），其中91个样本通过免疫表型分析来检测SARS-CoV-2特异性记忆T细胞反应。接种疫苗后，记忆B细胞和自然杀伤T淋巴细胞的水平增加。博罗罗村居民，女性,和Terena族成员在接种疫苗后有更高水平的抗尖峰IgG抗体，而酒精和烟草使用者的浓度较低。
结论：据我们所知，这是首次全面评估土著患者针对CoronaVac疫苗接种的抗体和T细胞反应.我们的发现表明，按照疫苗接种时间表，大多数患者都存在针对SARS-CoV-2的抗体反应和T细胞免疫。
