PDF(Pubmed)
Abstract：
The health benefits of young barley leaves, rich in dietary fiber, have been studied for several decades; however, their beneficial effects on the intestinal microenvironment remain to be elucidated. To investigate the effects of young barley leaf-derived dietary fiber (YB) on the gut microbiota and immunity, mice were fed an AIN-93G diet containing cellulose or YB and subjected to subsequent analysis. The population of MHC-II-positive conventional dendritic cells (cDCs) and CD86 expression in the cDCs of Peyer\'s patches were elevated in the YB-fed mice. MHC-II and CD86 expression was also elevated in the bone marrow-derived DCs treated with YB. 16S-based metagenomic analysis revealed that the gut microbiota composition was markedly altered by YB feeding. Among the gut microbiota, Lachnospiraceae, mainly comprising butyrate-producing NK4A136 spp., were overrepresented in the YB-fed mice. In fact, fecal butyrate concentration was also augmented in the YB-fed mice, which coincided with increased retinaldehyde dehydrogenase (RALDH) activity in the CD103+ cDCs of the mesenteric lymph nodes. Consistent with elevated RALDH activity, the population of colonic IgA+ plasma cells was higher in the YB-fed mice than in the parental control mice. In conclusion, YB has beneficial effects on the gut microbiota and intestinal immune system.
摘要：
大麦幼叶的健康益处，富含膳食纤维，已经研究了几十年；然而，它们对肠道微环境的有益作用仍有待阐明。探讨大麦幼叶源性膳食纤维(YB)对肠道菌群和免疫功能的影响。给小鼠喂食含有纤维素或YB的AIN-93G饮食并进行后续分析。在喂YB的小鼠中，MHC-II阳性常规树突状细胞（cDCs）的种群和Peyer的斑块cDCs中CD86的表达升高。在用YB处理的骨髓来源的DC中,MHC-II和CD86表达也升高。基于16S的宏基因组分析显示，YB喂养显着改变了肠道微生物群组成。在肠道微生物群中，落叶松科,主要包括产生丁酸的NK4A136。,在喂YB的小鼠中比例过高。事实上,YB喂养小鼠的粪便丁酸酯浓度也增加，这与肠系膜淋巴结CD103cDC中的视黄醛脱氢酶（RALDH）活性增加相吻合。与升高的RALDH活性一致，YB喂养的小鼠的结肠IgA浆细胞数量高于亲本对照小鼠。总之,YB对肠道微生物群和肠道免疫系统具有有益作用。
