PDF(Pubmed)
Abstract：
Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol-water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2\'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
摘要：
不知道它们的化学成分，许多文化已将牡丹根用于药用。用于人类治疗的现代植物修复需要有关牡丹根的化学及其生物活性的特定知识。在这项研究中,乙醇-水提取物是通过浸渍以及微波和超声辅助提取制备的(MAE和UAE，分别）为了从牡丹根中获得生物活性分子，PaeoniaperegrinaMill.,和塞尔维亚野生牡丹。化学表征;多酚和类黄酮含量;抗氧化剂,多抗酶，和提取物的抗菌活性；并进行了热水提取物的体外胃肠消化（GID）。在PT提取物中观察到最强的抗胆碱酯酶活性。在PP提取物中观察到最高的抗ABTS(2,2'-氮杂-双(3-乙基苯并噻唑啉-6-磺酸)自由基电位,而对于DPPH（2,2-二苯基-1-吡啶酰肼基），用PO提取物取得了最好的结果。关于抗菌活性，提取物对蜡样芽孢杆菌具有很强的效力。进行分子对接模拟以收集对多酚和其他芍药特异性分子在测试酶的活性位点中的结合亲和力和相互作用的见解。Paeonia茶的体外GID显示出单个生物活性物质的不同恢复和行为，没食子酸甲酯和二没食子酸甲酯的回收率增加，芍药苷及其衍生物的回收率降低。UAE和M获得的PT（Gulenovci）和PP（Pirot）提取物在大多数生物活性测定中更有效。这项研究代表了Paeonia根提取物在药学上可能应用的第一步，医学,和食品技术。
