PDF(Pubmed)
Abstract：
The present study aimed to investigate the differential effects of n-3 and n-6 polyunsaturated fatty acids (PUFAs) on placental and embryonic development. Pregnant mice were assigned to five groups: healthy control (HC), diabetes mellitus control (DMC), diabetes + low-dose n-3 PUFA (Ln-3), diabetes + high-dose n-3 PUFA (Hn-3), and diabetes + n-6 PUFA (n-6). On E12.5d, the Hn-3 group, but not the n-6 group, had a higher placenta weight. The weight ratio of embryo to placenta in the n-6 group was significantly lower than in the Hn-3 group but higher than in the DMC group. The Hn-3 group had significantly higher protein levels of VEGF, IGF-1, and IGFBP3, while the n-6 group had lower VEGF than the DMC group. Compared with the DMC group, embryonic Cer-16:0 was significantly higher in the Hn-3 group, while embryonic PC (36:6), PC (38:7), and PE (40:7) were significantly lower in the n-6 group. The embryo and placenta weights were positively correlated with placental VEGF, IGFBP3, and embryonic Cer-16:0, and they were negatively correlated with embryonic PC (36:6) and PE (40:7). The weight ratio of embryo to placenta was negatively correlated with embryonic PC (36:6). In addition, embryonic Cer-16:0 was positively correlated with placental VEGF and IGFBP3. In conclusion, n-3 PUFA and n-6 PUFA improved placental and embryonic growth through different mechanisms.
摘要：
本研究旨在研究n-3和n-6多不饱和脂肪酸（PUFAs）对胎盘和胚胎发育的差异影响。将怀孕小鼠分为五组：健康对照（HC），糖尿病控制（DMC），糖尿病+低剂量n-3PUFA（Ln-3），糖尿病+高剂量n-3PUFA（Hn-3），和糖尿病+n-6PUFA(n-6)。在E12.5d上，Hn-3组,但不是n-6组，胎盘重量较高。n-6组胚胎与胎盘的重量比明显低于Hn-3组，但高于DMC组。Hn-3组VEGF蛋白水平明显增高,IGF-1和IGFBP3，而n-6组VEGF低于DMC组。与DMC组相比，胚胎Cer-16:0在Hn-3组中显著增高,而胚胎PC（36：6），PC(38:7),在n-6组中，PE（40：7）显着降低。胚胎和胎盘重量与胎盘VEGF呈正相关,IGFBP3和胚胎Cer-16：0，它们与胚胎PC（36：6）和PE（40：7）呈负相关。胚胎与胎盘的重量比与胚胎PC（36：6）呈负相关。此外,胚胎Cer-16:0与胎盘VEGF、IGFBP3呈正相关。总之,n-3PUFA和n-6PUFA通过不同的机制改善胎盘和胚胎的生长。
