PDF(Pubmed)
Abstract：
The composition of microbiota in the digestive tract gut is essential for insect physiology, homeostasis, and pathogen infection. Little is known about the interactions between microbiota load and oral infection with baculoviruses. CnmeGV is an obligative baculovirus to Cnaphalocrocis medinalis. We investigated the impact of CnmeGV infection on the structure of intestinal microbes of C. medinalis during the initial infection stage. The results revealed that the gut microbiota profiles were dynamically driven by pathogen infection of CnmeGV. The numbers of all the OTU counts were relatively higher at the early and later stages, while the microbial diversity significantly increased early but dropped sharply following the infection. The compositional abundance of domain bacteria Firmicutes developed substantially higher. The significantly enriched and depleted species can be divided into four groups at the species level. Fifteen of these species were ultimately predicted as the biomarkers of CnmeGV infection. CnmeGV infection induces significant enrichment of alterations in functional genes related to metabolism and the immune system, encompassing processes such as carbohydrate, amino acid, cofactor, and vitamin metabolism. Finally, the study may provide an in-depth analysis of the relationship between host microbiota, baculovirus infection, and pest control of C. medinalis.
摘要：
消化道肠道微生物群的组成对昆虫生理至关重要，稳态，和病原体感染。关于微生物群负荷和杆状病毒口腔感染之间的相互作用知之甚少。CnmeGV是一种对稻长卷心菜的阴性杆状病毒。我们研究了CnmeGV感染在初始感染阶段对肠道微生物结构的影响。结果表明，肠道菌群分布是由CnmeGV的病原体感染动态驱动的。所有OTU计数的数量在早期和后期都相对较高，而微生物多样性在早期显着增加，但在感染后急剧下降。结构域细菌Firmicutes的组成丰度明显更高。显著富集和贫化的物种在物种水平上可分为四类。这些物种中的15种最终被预测为CnmeGV感染的生物标志物。CnmeGV感染诱导与代谢和免疫系统相关的功能基因的显著富集，包括碳水化合物等过程，氨基酸,辅因子，和维生素代谢。最后,这项研究可能会提供对宿主微生物群之间关系的深入分析，杆状病毒感染,和大黄梭菌的害虫防治。
