METHODS: The investigation methods were centered around non-invasive methods, such as biophoton emission (delayed fluorescence-DF, ultra-weak bioluminescence-UWLE), fluorescence induction (Fv/Fm) measurements, chlorophyll content estimation (SPAD) and vegetation index (NDVI) determination. Furthermore, the analytical determination of lipid oxidation (MDA level) and antioxidant capacity (FRAP) as well as gene expression studies of the antioxidative enzymes glutathione reductase (GR), glutathione S-transferase (GST) and lipoxygenase (LOX) for essential Zn and nonessential cadmium (Cd) were also carried out in order to clarify toxic symptoms through different Zn investigation approaches.
RESULTS: It was possible to identify a metabolic enhancement from 1000 µM; however, stress symptoms from the 2000 µM Zn treatment were noted for both the investigated photosynthetic and oxidative processes. The outcomes of this research contribute to the improvement of Zn mineral-supplementation technology, which is essential for maize growth, and the optimization of agricultural practices.
方法:调查方法围绕非侵入性方法,如生物光子发射(延迟荧光-DF,超弱生物发光-UWLE),荧光诱导(Fv/Fm)测量,叶绿素含量估算(SPAD)和植被指数(NDVI)测定。此外,脂质氧化(MDA水平)和抗氧化能力(FRAP)的分析测定以及抗氧化酶谷胱甘肽还原酶(GR)的基因表达研究,还对必需锌和非必需镉(Cd)进行了谷胱甘肽S-转移酶(GST)和脂氧合酶(LOX),以通过不同的锌调查方法阐明毒性症状。
结果:可以从1000µM确定代谢增强;但是,在研究的光合和氧化过程中,都注意到了2000µMZn处理的压力症状。这项研究的结果有助于锌矿物质补充技术的改进,这对玉米生长至关重要,和优化农业实践。