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Abstract：
Xinjiang brown cattle are highly resistant to disease and tolerant of roughage feeding. The identification of genes regulating mastitis resistance in Xinjiang brown cattle is a novel means of genetic improvement. In this study, the blood levels of IL-1β, IL-6, IL-10, TNF-α, and TGF-β in Xinjiang brown cattle with high and low somatic cell counts (SCCs) were investigated, showing that cytokine levels were higher in cattle with high SCCs. The peripheral blood transcriptomic profiles of healthy and mastitis-affected cattle were constructed by RNA-seq. Differential expression analysis identified 1632 differentially expressed mRNAs (DE-mRNAs), 1757 differentially expressed lncRNAs (DE-lncRNAs), and 23 differentially expressed circRNAs (DE-circRNAs), which were found to be enriched in key pathways such as PI3K/Akt, focal adhesion, and ECM-receptor interactions. Finally, ceRNA interaction networks were constructed using the differentially expressed genes and ceRNAs. It was found that keynote genes or mRNAs were also enriched in pathways such as PI3K-Akt, cholinergic synapses, cell adhesion molecules, ion binding, cytokine receptor activity, and peptide receptor activity, suggesting that the key genes and ncRNAs in the network may play an important role in the regulation of bovine mastitis.
摘要：
新疆褐牛抗病性强，耐粗饲料。新疆褐牛乳腺炎抗性基因的鉴定是一种新的遗传改良手段。在这项研究中,血液中IL-1β的水平，IL-6，IL-10，TNF-α，和TGF-β在新疆褐牛中的高和低体细胞计数（SCC）进行了调查，表明细胞因子水平在高SCC的牛中较高。通过RNA-seq构建健康牛和乳腺炎感染牛的外周血转录组学谱。差异表达分析鉴定了1632个差异表达的mRNA（DE-mRNA），1757个差异表达的lncRNAs（DE-lncRNAs），和23个差异表达的circRNAs(DE-circRNAs)，发现富含PI3K/Akt等关键途径，病灶粘连,和ECM-受体相互作用。最后,使用差异表达的基因和ceRNA构建了ceRNA相互作用网络。研究发现，主旨基因或mRNA也在PI3K-Akt等途径中富集，胆碱能突触，细胞粘附分子，离子结合，细胞因子受体活性，和肽受体活性，提示网络中的关键基因和ncRNAs可能在奶牛乳腺炎的调控中起重要作用。
