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Abstract：
The length of coleoptile is crucial for determining the sowing depth of oats in low-precipitation regions, which is significant for oat breeding programs. In this study, a diverse panel of 243 oat accessions was used to explore coleoptile length in two independent experiments. The panel exhibited significant variation in coleoptile length, ranging from 4.66 to 8.76 cm. Accessions from Africa, America, and the Mediterranean region displayed longer coleoptile lengths than those from Asia and Europe. Genome-wide association studies (GWASs) using 26,196 SNPs identified 34 SNPs, representing 32 quantitative trait loci (QTLs) significantly associated with coleoptile length. Among these QTLs, six were consistently detected in both experiments, explaining 6.43% to 10.07% of the phenotypic variation. The favorable alleles at these stable loci additively increased coleoptile length, offering insights for pyramid breeding. Gene Ontology (GO) analysis of the 350 candidate genes underlying the six stable QTLs revealed significant enrichment in cell development-related processes. Several phytochrome-related genes, including auxin transporter-like protein 1 and cytochrome P450 proteins, were found within these QTLs. Further validation of these loci will enhance our understanding of coleoptile length regulation. This study provides new insights into the genetic architecture of coleoptile length in oats.
摘要：
胚芽鞘的长度对于确定低降水地区燕麦的播种深度至关重要，这对燕麦育种计划意义重大。在这项研究中,在两个独立的实验中，使用了243种燕麦材料的不同小组来探索胚芽鞘长度。该组表现出胚芽鞘长度的显着变化，范围从4.66到8.76厘米。来自非洲的加入，美国,地中海地区的胚芽鞘长度比亚洲和欧洲的胚芽鞘长。使用26,196个SNP的全基因组关联研究（GWASs）鉴定了34个SNP，代表32个数量性状基因座（QTL）与胚芽鞘长度显着相关。在这些QTL中，在两个实验中都检测到了六个，解释6.43%至10.07%的表型变异。这些稳定基因座上的有利等位基因增加了胚芽鞘长度，为金字塔育种提供见解。对六个稳定QTL的350个候选基因的基因本体论（GO）分析揭示了细胞发育相关过程中的显着富集。几个与植物色素相关的基因，包括生长素转运蛋白1和细胞色素P450蛋白，在这些QTL中发现。这些基因座的进一步验证将增强我们对胚芽鞘长度调节的理解。这项研究为燕麦胚芽鞘长度的遗传结构提供了新的见解。
