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Abstract：
Background and Objectives: There are many surgical techniques for oroantral communication treatment, one of which is the buccal fat pad. Of particular interest is the high reparative potential of the buccal fat pad, which may be contributed to by the presence of mesenchymal stem cells. The purpose of this work is to evaluate the reparative potential of BFP cells using morphological and immunohistochemical examination. Materials and Methods: 30 BFP samples were provided by the Clinic of Maxillofacial and Plastic Surgery of the Russian University of Medicine (Moscow, Russia) from 28 patients. Morphological examination of 30 BFP samples was performed at the Institute of Clinical Morphology and Digital Pathology of Sechenov University. Hematoxylin-eosin, Masson trichrome staining and immunohistochemical examination were performed to detect MSCs using primary antibodies CD133, CD44 and CD10. Results: During staining with hematoxylin-eosin and Masson\'s trichrome, we detected adipocytes of white adipose tissue united into lobules separated by connective tissue layers, a large number of vessels of different calibers, as well as the general capsule of BFP. The thin connective tissue layers contained neurovascular bundles. Statistical processing of the results of the IHC examination of the samples using the Mann-Whitney criterion revealed that the total number of samples in which the expression of CD44, CD10 and CD133 antigens was confirmed was statistically significantly higher than the number of samples where the expression was not detected (p < 0.05). Conclusions: During the morphological study of the BFP samples, we revealed statistically significant signs of MSCs presence (p < 0.05), including in the brown fat tissue, which proves the high reparative potential of this type of tissue and can make the BFP a choice option among other autogenous donor materials when eliminating OAC and other surgical interventions in the maxillofacial region.
摘要：
背景与目的:口腔沟通治疗的外科技术很多,其中之一是颊脂肪垫。特别令人感兴趣的是颊脂肪垫的高修复潜力，这可能是由间充质干细胞的存在造成的。这项工作的目的是使用形态学和免疫组织化学检查评估BFP细胞的修复潜力。材料和方法：30个BFP样本由俄罗斯医科大学颌面整形外科诊所提供（莫斯科，俄罗斯）来自28名患者。在Sechenov大学临床形态学和数字病理学研究所对30个BFP样品进行了形态学检查。苏木精-伊红,使用一级抗体CD133，CD44和CD10进行Masson三色染色和免疫组织化学检查以检测MSC。结果：在用苏木精-伊红和Masson三色染色期间，我们检测到白色脂肪组织的脂肪细胞被结缔组织层分离成小叶，大量不同口径的船只，以及BFP的一般胶囊。薄的结缔组织层包含神经血管束。使用Mann-Whitney标准对样品的IHC检查结果进行统计处理,显示其中CD44、CD10和CD133抗原的表达被确认的样品的总数在统计学上显著高于其中未检测到表达的样品的数量(p<0.05)。结论：在BFP样品的形态学研究中，我们发现MSCs存在有统计学意义（p<0.05），包括棕色脂肪组织，这证明了这种组织的高修复潜力，并且在消除颌面部区域的OAC和其他外科手术时，可以使BFP成为其他自体供体材料中的选择。
