PDF(Pubmed)
Abstract：
The gradual deterioration of articular cartilage was thought to be the central event in osteoarthritis (OA), but recent studies demonstrated the importance of low-grade synovitis in the progression of OA. The Syndecan (SDC) family of membrane proteoglycans is known to be involved in the regulation of inflammation, but there is limited evidence considering the role of syndecans in OA synovitis. Our study aimed to investigate the hip OA synovial membrane expression patterns of SDC1, SDC2 and SDC4, as well as exostosins and sulfotransferases (enzymes involved in the polymerisation and modification of syndecans\' heparan sulphate chains). Synovial membrane samples of patients with OA (24) were divided into two groups according to their Krenn synovitis score severity. The immunohistochemical expressions of SDC1, SDC2, SDC4, EXT1, EXT2, NDST1 and NDST2 in synovial intima and subintima were then analysed and compared with the control group (patients with femoral neck fracture). According to our study, the immunoexpression of SDC1, NDST1 and EXT2 is significantly increased in the intimal cells of OA synovial membrane in patients with lower histological synovitis scores and SDC4 in patients with higher synovitis scores, in comparison with non-OA controls. The difference in the expression of SDC2 among the OA and non-OA groups was insignificant. SDC1, SDC4, NDST1 and EXT2 seem to be involved as inflammation moderators in low-grade OA synovitis and, therefore, should be further investigated as potential markers of disease progression and therapeutic goals.
摘要：
关节软骨的逐渐恶化被认为是骨关节炎（OA）的中心事件，但最近的研究证明了低度滑膜炎在OA进展中的重要性.已知膜蛋白聚糖的Syndecan（SDC）家族参与炎症的调节，但是考虑syndecans在OA滑膜炎中的作用的证据有限。我们的研究旨在研究SDC1，SDC2和SDC4的髋关节OA滑膜表达模式，以及外植体蛋白酶和磺基转移酶（参与聚合和修饰的酶）。将患有OA(24)的患者的滑膜样品根据他们的Krenn滑膜炎评分严重程度分为两组。然后分析SDC1，SDC2，SDC4，EXT1，EXT2，NDST1和NDST2在滑膜内膜和内膜下的免疫组化表达，并与对照组（股骨颈骨折患者）进行比较。根据我们的研究,SDC1、NDST1和EXT2的免疫表达在组织学滑膜炎评分较低的患者OA滑膜内膜细胞中显著升高,与非OA对照相比。SDC2在OA组和非OA组之间的表达差异不显著。SDC1，SDC4，NDST1和EXT2似乎参与了低级别OA滑膜炎的炎症调节剂，因此,应进一步研究作为疾病进展和治疗目标的潜在标志物。
