PDF(Pubmed)
Abstract：
Due to the widespread use of shellfish ingredients in food products, accurate food labelling is urgently needed for consumers with shellfish allergies. Most crustacean allergen detection systems target the immunorecognition of the allergenic protein tropomyosin. However, this mode of detection may be affected by an origin-dependent protein composition. This study determined if the geographic location of capture, or aquaculture, influenced the allergenic protein profiles of Black Tiger Shrimp (Penaeus monodon), one of the most farmed and consumed shrimp species worldwide. Protein composition was analysed in shrimp from nine different locations in the Asia-Pacific by SDS-PAGE, immunoblotting, and mass spectrometry. Ten of the twelve known shrimp allergens were detected, but with considerable differences between locations. Sarcoplasmic calcium-binding protein, myosin light chain, and tropomyosin were the most abundant allergens in all locations. Hemocyanin-specific antibodies could identify up to six different isoforms, depending on the location of origin. Similarly, tropomyosin abundance varied by up to 13 times between locations. These findings suggest that allergen abundance may be related to shrimp origin and, thus, shrimp origin might directly impact the readout of commercial crustacean allergen detection kits, most of which target tropomyosin, and this should be considered in food safety assessments.
摘要：
由于贝类成分在食品中的广泛使用，对贝类过敏的消费者迫切需要准确的食品标签。大多数甲壳动物过敏原检测系统的目标是过敏原原肌球蛋白的免疫识别。然而,这种检测模式可能受到来源依赖性蛋白质组成的影响.这项研究确定了捕获的地理位置，或水产养殖，影响了黑虎虾（斑节对虾）的致敏蛋白谱，世界上养殖和消费最多的虾之一。通过SDS-PAGE分析了来自亚太地区九个不同地点的虾的蛋白质组成，免疫印迹,和质谱。检测到12种已知虾过敏原中的10种，但是位置之间有很大的差异。肌浆钙结合蛋白,肌球蛋白轻链,原肌球蛋白是所有地区最丰富的过敏原。血蓝蛋白特异性抗体可以鉴定多达六种不同的亚型，取决于原点的位置。同样，原肌球蛋白的丰度在不同位置之间变化多达13倍。这些发现表明，过敏原的丰度可能与虾的起源有关，因此，虾的来源可能直接影响商业甲壳类过敏原检测试剂盒的读数，其中大部分靶向原肌球蛋白，这应该在食品安全评估中考虑。
