PDF(Pubmed)
Abstract：
\"Basal-like\" (BL) morphology and the expression of cancer testis antigens (CTA) in breast cancer still have unclear prognostic significance. The aim of our research was to explore correlations of the morphological characteristics and tumor microenvironment in triple-negative breast carcinomas (TNBCs) with multi-MAGE-A CTA expression and to determine their prognostic significance. Clinical records of breast cancer patients who underwent surgery between January 2017 and December 2018 in four major Croatian clinical centers were analyzed. A total of 97 non-metastatic TNBCs with available tissue samples and treatment information were identified. Cancer tissue sections were additionally stained with programmed death-ligand 1 (PD-L1) Ventana (SP142) and multi-MAGE-A (mAb 57B). BL morphology was detected in 47 (49%) TNBCs and was associated with a higher Ki-67 proliferation index and histologic grade. Expression of multi-MAGE-A was observed in 77 (79%) TNBCs and was significantly associated with BL morphology. Lymphocyte-predominant breast cancer (LPBC) status was detected in 11 cases (11.3%) and significantly correlated with the Ki-67 proliferation index, increased number of intratumoral lymphocytes (itTIL), and PD-L1 expression. No impact of BL morphology, multi-MAGE-A expression, histologic type, or LPBC status on disease-free survival was observed. Our data suggest that tumor morphology could help identify patients with potential benefits from CTA-targeting immunotherapy.
摘要：
乳腺癌的“基底样”（BL）形态和癌睾丸抗原（CTA）的表达仍不清楚其预后意义。我们研究的目的是探讨三阴性乳腺癌（TNBC）的形态学特征和肿瘤微环境与多MAGE-ACTA表达的相关性，并确定其预后意义。分析了2017年1月至2018年12月在克罗地亚四个主要临床中心接受手术的乳腺癌患者的临床记录。鉴定了总共97个具有可用组织样品和治疗信息的非转移性TNBC。另外用程序性死亡-配体1(PD-L1)Ventana(SP142)和多MAGE-A(mAb57B)对癌组织切片进行染色。在47（49％）TNBC中检测到BL形态，并与较高的Ki-67增殖指数和组织学分级有关。在77（79％）TNBC中观察到多MAGE-A的表达，并且与BL形态显着相关。11例（11.3％）检测到淋巴细胞占优势的乳腺癌（LPBC）状态，并与Ki-67增殖指数显着相关。肿瘤内淋巴细胞（itTIL）数量增加，和PD-L1表达。BL形态无影响,多MAGE-A表达式,组织学类型，或LPBC状态对无病生存的影响。我们的数据表明，肿瘤形态学可以帮助识别具有CTA靶向免疫疗法潜在益处的患者。
