PDF(Pubmed)
Abstract：
This study presents an evaluation of seventeen newly produced recombinant trivalent chimeric proteins (containing the same immunodominant fragment of SAG1 and SAG2 of Toxoplasma gondii antigens, and an additional immunodominant fragment of one of the parasite antigens, such as AMA1, GRA1, GRA2, GRA5, GRA6, GRA7, GRA9, LDH2, MAG1, MIC1, MIC3, P35, and ROP1) as a potential alternative to the whole-cell tachyzoite lysate (TLA) used in the detection of infection in small ruminants. These recombinant proteins, obtained by genetic engineering and molecular biology methods, were tested for their reactivity with specific anti-Toxoplasma IgG antibodies contained in serum samples of small ruminants (192 samples of sheep serum and 95 samples of goat serum) using an enzyme-linked immunosorbent assay (ELISA). The reactivity of six recombinant trivalent chimeric proteins (SAG1-SAG2-GRA5, SAG1-SAG2-GRA9, SAG1-SAG2-MIC1, SAG1-SAG2-MIC3, SAG1-SAG2-P35, and SAG1-SAG2-ROP1) with IgG antibodies generated during T. gondii invasion was comparable to the sensitivity of TLA-based IgG ELISA (100%). The obtained results show a strong correlation with the results obtained for TLA. This suggests that these protein preparations may be a potential alternative to TLA used in commercial tests and could be used to develop a cheaper test for the detection of parasite infection in small ruminants.
摘要：
这项研究对17种新产生的重组三价嵌合蛋白（包含弓形虫抗原的SAG1和SAG2的相同免疫显性片段，和一种寄生虫抗原的额外免疫显性片段，例如AMA1，GRA1，GRA2，GRA5，GRA6，GRA7，GRA9，LDH2，MAG1，MIC1，MIC3，P35和ROP1）作为全细胞速殖子裂解物（TLA）的潜在替代品用于检测小反刍动物的感染。这些重组蛋白，通过基因工程和分子生物学方法获得，使用酶联免疫吸附测定（ELISA）测试了它们与小反刍动物血清样品（192个绵羊血清样品和95个山羊血清样品）中包含的特异性抗弓形虫IgG抗体的反应性。六种重组三价嵌合蛋白(SAG1-SAG2-GRA5、SAG1-SAG2-GRA9、SAG1-SAG2-MIC1、SAG1-SAG2-MIC3、SAG1-SAG2-P35和SAG1-SAG2-ROP1)与弓形虫入侵期间产生的IgG抗体的反应性与基于TLA的IgG的敏感性相当(100%)。获得的结果显示与TLA获得的结果具有很强的相关性。这表明这些蛋白质制剂可能是商业测试中使用的TLA的潜在替代品，并可用于开发一种更便宜的测试，用于检测小反刍动物中的寄生虫感染。
