PDF(Pubmed)
Abstract：
C-TERMINALLY ENCODED PEPTIDEs (CEPs) are a class of peptide hormones that have been shown in previous studies to play an important role in regulating the development and response to abiotic stress in model plants. However, their role in cotton is not well understood. In this study, we identified 54, 59, 34, and 35 CEP genes from Gossypium hirsutum (2n = 4x = 52, AD1), G. barbadense (AD2), G. arboreum (2n = 2X = 26, A2), and G. raimondii (2n = 2X = 26, D5), respectively. Sequence alignment and phylogenetic analyses indicate that cotton CEP proteins can be categorized into two subgroups based on the differentiation of their CEP domain. Chromosomal distribution and collinearity analyses show that most of the cotton CEP genes are situated in gene clusters, suggesting that segmental duplication may be a critical factor in CEP gene expansion. Expression pattern analyses showed that cotton CEP genes are widely expressed throughout the plant, with some genes exhibiting specific expression patterns. Ectopic expression of GhCEP46-D05 in Arabidopsis led to a significant reduction in both root length and seed size, resulting in a dwarf phenotype. Similarly, overexpression of GhCEP46-D05 in cotton resulted in reduced internode length and plant height. These findings provide a foundation for further investigation into the function of cotton CEP genes and their potential role in cotton breeding.
摘要：
C-末端编码肽(CEP)是一类肽类激素,其在先前的研究中已显示在调节模型植物的发育和对非生物胁迫的响应中起重要作用。然而,它们在棉花中的作用还没有得到很好的理解。在这项研究中,我们从陆地棉（2n=4x=52，AD1）中鉴定了54、59、34和35个CEP基因，G.巴巴多斯（AD2），植物园(2n=2X=26，A2)，和G.raimondii(2n=2X=26，D5)，分别。序列比对和系统发育分析表明，棉花CEP蛋白可以根据其CEP结构域的分化分为两个亚组。染色体分布和共线性分析表明，棉花CEP基因大部分位于基因簇中，提示分段复制可能是CEP基因扩增的关键因素。表达模式分析表明，棉花CEP基因在整个植物中广泛表达，一些基因表现出特定的表达模式。GhCEP46-D05在拟南芥中的异位表达导致根长和种子大小的显着减少，导致矮人表型。同样，GhCEP46-D05在棉花中的过表达导致节间长度和株高降低。这些发现为进一步研究棉花CEP基因的功能及其在棉花育种中的潜在作用奠定了基础。
