PDF(Pubmed)
Abstract：
The high content of bioactive compounds in Aronia melanocarpa fruit offers health benefits. In this study, the anti-atherosclerotic effect of Aronia extracts was assessed. The impact on the level of adhesion molecules and the inflammatory response of human umbilical vein endothelial cells (HUVECs) was shown in relation to the chemical composition and the stage of ripening of the fruits. Samples were collected between May (green, unripe) and October (red, overripe) on two farms in Poland, which differed in climate. The content of chlorogenic acids, anthocyanins, and carbohydrates in the extracts was determined using HPLC-DAD/RI. The surface expression of ICAM-1 and VCAM-1 in HUVECs was determined by flow cytometry. The mRNA levels of VCAM-1, ICAM-1, IL-6, and MCP-1 were assessed using the quantitative real-time PCR method. The farms\' geographical location was associated with the quantity of active compounds in berries and their anti-atherosclerotic properties. Confirmed activity for green fruits was linked to their high chlorogenic acid content.
摘要：
黑果Aronia果实中的高含量生物活性化合物提供健康益处。在这项研究中,评估了Aronia提取物的抗动脉粥样硬化作用。显示了对人脐静脉内皮细胞（HUVEC）的粘附分子水平和炎症反应的影响与果实的化学成分和成熟阶段有关。在5月之间收集样本(绿色，未成熟)和十月(红色，过熟)在波兰的两个农场，气候不同。绿原酸的含量，花青素,使用HPLC-DAD/RI测定提取物中的碳水化合物。流式细胞术检测HUVECs中ICAM-1和VCAM-1的表面表达。使用定量实时PCR方法评估VCAM-1，ICAM-1，IL-6和MCP-1的mRNA水平。农场的地理位置与浆果中活性化合物的数量及其抗动脉粥样硬化特性有关。绿色水果的确认活性与其高绿原酸含量有关。
