PDF(Pubmed)
Abstract：
The Postmortem Interval (PMI) is the time from the death of an animal to its discovery. From a veterinary forensic standpoint, an accurate estimation of the PMI is of particular importance, especially with the observed increase in deaths of domestic and wild animals. A preliminary study was conducted using the eyes of domestic pigs. A biochemical analysis was conducted on the vitreous humor of the eye, whilst a histological analysis was conducted on the retina. The eyes were stored at +4 °C and changes were assessed at time intervals of 0, 12, 24, 48, and 120 h. The biochemical analysis during the PMI established a decrease in sodium, chlorine, and glucose concentrations, and a rise in potassium concentration. Accordingly, a simple linear regression showed a significant correlation between changes in concentrations of sodium (Na+), potassium (K+), chloride (Cl-), and glucose, in relation to the PMI. The histological analysis showed evident morphological changes in the retina, which included homogenization of the rod and cone cells, pyknosis of the outer nuclear layer, homogenization of the outer plexiform layer, pyknosis of the inner nuclear layer, homogenization of the inner plexiform layer, and pyknosis of the nuclei of the ganglion layer of the retina.
摘要：
死后间隔（PMI）是从动物死亡到发现动物的时间。从兽医法医的角度来看,对PMI的准确估计尤为重要，特别是观察到家畜和野生动物死亡人数的增加。使用家猪的眼睛进行了初步研究。对眼睛的玻璃体液进行了生化分析，同时对视网膜进行组织学分析。将眼睛储存在+4°C，并以0、12、24、48和120小时的时间间隔评估变化。PMI期间的生化分析确定钠减少，氯,和葡萄糖浓度，钾浓度上升.因此，简单的线性回归显示钠(Na+)浓度变化之间存在显著相关性，钾（K+），氯化物(Cl-),和葡萄糖，关于PMI。组织学分析显示视网膜有明显的形态学改变,其中包括视杆和视锥细胞的匀浆，外核层的固缩，外丛状层的均质化，内部核层的固缩，内丛状层的均质化，和视网膜神经节层细胞核的固缩。
