PDF(Pubmed)
Abstract：
This study investigated six-month outcomes of first models of ascending aortic replacement. The molds used to produce the Biotube were implanted subcutaneously in goats. After 2-3 months, the molds were explanted to obtain the Biotubes (inner diameter, 12 mm; wall thickness, 1.5 mm). Next, we performed ascending aortic replacement using the Biotube in five allogenic goats. At 6 months, the animals underwent computed tomography (CT) and histologic evaluation. As a comparison, we performed similar surgeries using glutaraldehyde-fixed autologous pericardial rolls or pig-derived heterogenous Biotubes. At 6 months, CT revealed no aneurysmalization of the Biotube or pseudoaneurysm formation. The histologic evaluation showed development of endothelial cells, smooth muscle cells, and elastic fibers along the Biotube. In the autologous pericardium group, there was no evidence of new cell development, but there was calcification. The histologic changes observed in the heterologous Biotube group were similar to those in the allogenic Biotube group. However, there was inflammatory cell infiltration in some heterologous Biotubes. Based on the above, we could successfully create the world\'s first Biotube-based ascending aortic replacement models. The present results indicate that the Biotube may serve as a scaffold for aortic tissue regeneration.
摘要：
这项研究调查了升主动脉置换的第一个模型的六个月结果。将用于生产Biotube的模具皮下植入山羊中。2-3个月后,将模具外植以获得生物管(内径，12毫米;壁厚,1.5mm)。接下来,我们使用Biotube对5只同种山羊进行了升主动脉置换术.6个月时,动物接受计算机断层扫描(CT)和组织学评估.作为比较,我们使用戊二醛固定的自体心包卷或猪源异质生物管进行了类似的手术.6个月时,CT显示Biotube没有动脉瘤形成或假性动脉瘤形成。组织学评估显示内皮细胞发育，平滑肌细胞，和沿着Biotube的弹性纤维。在自体心包组中，没有新细胞发育的证据,但有钙化.在异源Biotube组中观察到的组织学变化与同种异体Biotube组相似。然而,一些异源生物管有炎性细胞浸润。根据上述情况,我们可以成功创建世界上第一个基于Biotube的升主动脉置换模型.目前的结果表明Biotube可以作为主动脉组织再生的支架。
