PDF(Pubmed)
Abstract：
Venous leg ulcers are one of the most common nonhealing conditions and represent an important clinical problem. The application of pulsed radiofrequency electromagnetic fields (PRF-EMFs), already applied for pain, inflammation, and new tissue formation, can represent a promising approach for venous leg ulcer amelioration. This study aims to evaluate the effect of PRF-EMF exposure on the inflammatory, antioxidant, cell proliferation, and wound healing characteristics of human primary dermal fibroblasts collected from venous leg ulcer patients. The cells\' proliferative and migratory abilities were evaluated by means of a BrdU assay and scratch assay, respectively. The inflammatory response was investigated through TNFα, TGFβ, COX2, IL6, and IL1β gene expression analysis and PGE2 and IL1β production, while the antioxidant activity was tested by measuring GSH, GSSG, tGSH, and GR levels. This study emphasizes the ability of PRF-EMFs to modulate the TGFβ, COX2, IL6, IL1β, and TNFα gene expression in exposed ulcers. Moreover, it confirms the improvement of the proliferative index and wound healing ability presented by PRF-EMFs. In conclusion, exposure to PRF-EMFs can represent a strategy to help tissue repair, regulating mediators involved in the wound healing process.
摘要：
下肢静脉溃疡是最常见的不愈合病症之一，是重要的临床问题。脉冲射频电磁场（PRF-EMFs）的应用，已经申请了疼痛，炎症,和新的组织形成，可以代表改善静脉性腿部溃疡的有希望的方法。本研究旨在评估PRF-EMF暴露对炎症的影响,抗氧化剂，细胞增殖，和从静脉性腿部溃疡患者收集的人原代真皮成纤维细胞的伤口愈合特征。通过BrdU分析和划痕分析评估细胞的增殖和迁移能力，分别。通过TNFα研究炎症反应，TGFβ,COX2，IL6和IL1β基因表达分析以及PGE2和IL1β的产生，而抗氧化活性是通过测量GSH来测试的，GSSG,tGSH,和GR水平。这项研究强调了PRF-EMFs调节TGFβ的能力，COX2,IL6,IL1β,和TNFα基因在暴露性溃疡中的表达。此外，它证实了PRF-EMFs呈现的增殖指数和伤口愈合能力的改善。总之,暴露于PRF-EMFs可以代表一种帮助组织修复的策略，参与伤口愈合过程的调节介质。
