PDF(Pubmed)
Abstract：
Toll-like receptor 9 (TLR-9) is a protein that helps our immune system identify specific DNA types. Upon detection, CpG oligodeoxynucleotides signal the immune system to generate cytokines, essential proteins that contribute to the body\'s defence against infectious diseases. Native phosphodiester type B CpG ODNs induce only Interleukin-6 with no effect on interferon-α. We prepared silicon quantum dots containing different surface charges, such as positive, negative, and neutral, using amine, acrylate-modified Plouronic F-127, and Plouronic F-127. Then, class B CpG ODNs are loaded on the surface of the prepared SiQDs. The uptake of ODNs varies based on the surface charge; positively charged SiQDs demonstrate higher adsorption compared to SiQDs with negative and neutral surface charges. The level of cytokine production in peripheral blood mononuclear cells was found to be associated with the surface charge of SiQDs prior to the binding of the CpG ODNs. Significantly higher levels of IL-6 and IFN-α induction were observed compared to neutral and negatively charged SiQDs loaded with CpG ODNs. This observation strongly supports the notion that the surface charge of SiQDs effectively regulates cytokine induction.
摘要：
Toll样受体9（TLR-9）是一种帮助我们的免疫系统识别特定DNA类型的蛋白质。一旦检测到,CpG寡脱氧核苷酸信号免疫系统产生细胞因子，有助于人体防御传染病的必需蛋白质。天然磷酸二酯B型CpGODN仅诱导白细胞介素-6，对干扰素-α无影响。我们制备了含有不同表面电荷的硅量子点，如积极的，负,中立,使用胺，丙烯酸酯改性的PlouronicF-127和PlouronicF-127。然后，B类CpGODN负载在制备的SiQD的表面上。ODN的吸收基于表面电荷而变化;与具有负和中性表面电荷的SiQD相比,带正电荷的SiQD表现出更高的吸附。发现外周血单核细胞中细胞因子产生的水平与CpGODN结合之前SiQD的表面电荷相关。与负载有CpGODN的中性和带负电荷的SiQD相比,观察到显著更高水平的IL-6和IFN-α诱导。该观察结果强烈支持SiQD的表面电荷有效调节细胞因子诱导的观点。
