Abstract：
The number of poisoning cases caused by the Lepiota genus is globally increasing. This genus has more poisonous species than the Amanita genus, and many Lepiota species can cause severe toxicity and death if ingested. As recognized in the literature, L. castanea is a toxic species containing amatoxin. Although crude analytical methods have shown that L. castanea contains amatoxins, more recent and sensitive analyses suggest otherwise. Toxin concentrations can vary even among the same fungal species due to geographical and climatic differences. Therefore, this confusion can be resolved by analyzing L. castanea toxins from different geographical regions. This study aimed to demonstrate the toxin levels of L. castanea collected from forests in different regions of Turkiye (Istanbul and Kocaeli) using sensitive methods. The collected mushrooms were analyzed for alpha amanitin, beta amanitin, gamma amanitin, amanin, phallacidin, and phalloidin levels using RP-HPLC-UV and LC-ESI-MS/MS methods. L. castanea mushroom was found to be free of amatoxin and phallotoxin. Our study revealed for the first time that L. castanea mushrooms from different geographical regions of Turkiye do not contain amatoxin and phallotoxin. Supporting these findings with new studies from different parts of the world would be appropriate.
摘要：
由Lepiota属引起的中毒病例在全球范围内增加。这个属比天牛属有更多的有毒物种，许多Lepiota物种如果摄入会导致严重的毒性和死亡。正如文献所承认的那样，蓖麻乳杆菌是含有amatoxin的有毒物种。尽管粗略的分析方法表明，蓖麻乳杆菌含有阿马毒素，最近的和敏感的分析表明，情况并非如此。由于地理和气候差异，即使在相同的真菌物种中，毒素浓度也会有所不同。因此,这种困惑可以通过分析来自不同地理区域的蓖麻乳杆菌毒素来解决。这项研究旨在证明使用敏感方法从Turkiye（伊斯坦布尔和Kocaeli）不同地区的森林中收集的蓖麻乳杆菌的毒素水平。分析收集的蘑菇的α-amanitin，β-amanitin,γ-amanitin,amanin,phallacidin,和使用RP-HPLC-UV和LC-ESI-MS/MS方法的连环肽水平。发现栗树蘑菇不含amatoxin和phallotoxin。我们的研究首次揭示了来自Turkiye不同地理区域的栗树蘑菇不含有amatoxin和phallotoxin。用来自世界不同地区的新研究来支持这些发现是合适的。
