Abstract：
Cell culturing is a cornerstone of tissue engineering, playing a crucial role in tissue regeneration, drug screening, and the study of disease mechanisms. Among various culturing techniques, 3D culture systems, particularly those utilizing suspended fiber scaffolds, offer a more physiologically relevant environment than traditional 2D monolayer cultures. These 3D scaffolds enhance cell growth, differentiation, and proliferation by mimicking the in vivo cellular milieu. This review focuses on the critical role of suspended fiber scaffolds in tissue engineering. We compare the effectiveness of 3D suspended fiber scaffolds with 2D culture systems, discussing their respective benefits and limitations in the context of tissue regeneration. Furthermore, we explore the preparation methods of suspended fiber scaffolds and their potential applications. The review concludes by considering future research directions for optimizing suspended fiber scaffolds to address specific challenges in tissue regeneration, underscoring their significant promise in advancing tissue engineering and regenerative medicine.
摘要：
细胞培养是组织工程的基石,在组织再生中起着至关重要的作用，药物筛选,和疾病机制的研究。在各种培养技术中，3D文化系统，特别是那些利用悬浮纤维支架的，提供比传统2D单层培养更生理相关的环境。这些3D支架增强了细胞生长，分化,通过模拟体内细胞环境进行增殖。本文综述了悬浮纤维支架在组织工程中的关键作用。我们比较了3D悬浮纤维支架与2D培养系统的有效性，讨论它们在组织再生方面的各自益处和局限性。此外，探讨了悬浮纤维支架的制备方法及其潜在应用。该综述最后考虑了未来的研究方向，以优化悬浮纤维支架，以解决组织再生中的具体挑战。强调了他们在推进组织工程和再生医学方面的重要前景。
