Abstract:
The Wooden Breast myopathy results in the necrosis and fibrosis of breast muscle fibers in fast-growing heavy weight meat-type broiler chickens. Myogenic satellite cells are required to repair and regenerate the damaged muscle fibers. Using Genome Wide Association, candidate genes affected with Wooden Breast have been previously reported. The effect of these genes on satellite cell proliferation, differentiation, and the synthesis of lipids by satellite cells is unknown. Satellite cells isolated from the pectoralis major muscle from commercial Ross 708 broilers and a Randombred chicken (RBch) line were used. Expression of calponin 1 (CNN1) and PHD and ring fingers domains 1 (PHRF1) were knocked down by silent interfering RNA to determine their effect on satellite cell-mediated proliferation, differentiation, and lipid accumulation. CNN1 and PHRF1 affected satellite cell activity and lipid accumulation in both lines. Proliferation was reduced in the Ross 708 and RBch lines by knocking down the expression of both genes, and differentiation was affected with a line and treatment interaction when gene expression was reduced at the beginning of proliferation. During differentiation lipid accumulation was decreased with knocking down the expression of CNN1 and PHRF1. Both CNN1 and PHRF1 have not been reported previously in skeletal muscle and further research is required to determine their effect on satellite cell-mediated growth and regeneration of the pectoralis major (breast) muscle.
摘要:
木乳肌病导致快速生长的重体重肉型肉鸡的乳腺肌纤维坏死和纤维化。需要成肌卫星细胞来修复和再生受损的肌纤维。利用全基因组关联,先前已经报道了受木胸影响的候选基因。这些基因对卫星细胞增殖的影响,分化,卫星细胞合成的脂质是未知的。使用从商业Ross708肉鸡的胸大肌分离的卫星细胞和随机繁殖的鸡(RBch)系。通过沉默干扰RNA降低钙蛋白1(CNN1)和PHD和环指结构域1(PHRF1)的表达,以确定它们对卫星细胞介导的增殖的影响,分化,和脂质积累。CNN1和PHRF1影响两个系中的卫星细胞活性和脂质积累。通过敲低这两个基因的表达,Ross708和RBch系的增殖减少,当增殖开始时基因表达降低时,分化受到品系和处理相互作用的影响。在分化过程中,随着CNN1和PHRF1表达的降低,脂质积累减少。CNN1和PHRF1以前在骨骼肌中都没有报道,需要进一步的研究来确定它们对卫星细胞介导的生长和胸大肌(乳房)再生的影响。