PDF(Pubmed)
Abstract：
People have difficulty in eating and speaking when they are suffering from mouth ulcers. Some studies suggest that estradiol is associated with the development and treatment of mouth ulcers, while some do not. To clarify the effect of estradiol on mouth ulcers, we performed 2-sample Mendelian randomization and multivariable Mendelian randomization (MVMR) analysis to evaluate their relationship. Data were obtained from the IEU OpenGWAS project and UK biobank, including male estradiol dataset (case/controls = 13,367/134,323), female estradiol dataset (case/controls = 37,461/126,524), mouth ulcers dataset (case/controls = 47,102/414,011). The causal associations were estimated by MR-Egger, weighted median, inverse-variance weighted (IVW) method, simple mode, and weighted mode. Cochran Q test, MR-Egger intercept test, MR-PRESSO tests, and leave-one-out analysis were used to examine sensitivity analyses. The MVMR controlling for depression, anxiety or panic attacks, severe stress and adjustment disorders was used to assess the effect of estradiol on mouth ulcers. Through screening, 13 single nucleotide polymorphisms (SNPs) of males and 2 SNPs of females in estradiol were used for harmonizing and MR analysis. The 2-sample MR analysis showed no causal association between estradiol of males and mouth ulcers (IVW, OR: 0.998, 95% confidence interval [95% CI]: 0.995-1.001, P = .18). Similar results were obtained between estradiol of females and mouth ulcers (IVW, OR: 1.000, 95% CI: 0.988-1.012, P = .97). No pleiotropy and heterogeneity were found and the results were robust (P > .05). After adjusting for the potential effects of confounders, estradiol of males and mouth ulcers still showed no causal association through MVMR analysis (P = .081). While MVMR analysis showed that the causal relationship between estradiol and mouth ulcers in women could not be statistical for the small number of SNPs. There was no evidence of a causal relationship between estradiol and mouth ulcers. The strategy of treating mouth ulcers with estradiol still needs to be confirmed by more studies.
摘要：
当人们患有口腔溃疡时，他们难以进食和说话。一些研究表明，雌二醇与口腔溃疡的发展和治疗有关，而有些人没有。为了阐明雌二醇对口腔溃疡的影响，我们进行了2个样本孟德尔随机化和多变量孟德尔随机化(MVMR)分析,以评估它们之间的关系.数据来自IEUOpenGWAS项目和英国生物银行，包括男性雌二醇数据集（病例/对照=13,367/134,323），女性雌二醇数据集(病例/对照=37,461/126,524)，口腔溃疡数据集（病例/对照=47,102/414,011）。因果关联由MR-Egger估计，加权中位数，逆方差加权(IVW)方法，简单模式，和加权模式。CochranQ测试,MR-Egger截距测试,MR-PRESSO测试,和留一法分析用于检查敏感性分析。控制抑郁症的MVMR，焦虑或恐慌症发作，使用严重的应激和调节障碍来评估雌二醇对口腔溃疡的影响.通过筛选,雌二醇中男性的13个单核苷酸多态性（SNP）和女性的2个SNP用于协调和MR分析。2个样本的MR分析显示，男性的雌二醇与口腔溃疡之间没有因果关系（IVW，OR：0.998，95%置信区间[95%CI]：0.995-1.001，P=.18）。在女性的雌二醇和口腔溃疡之间获得了类似的结果(IVW，OR：1.000，95%CI：0.988-1.012，P=0.97）。没有发现多效性和异质性，结果是稳健的（P>0.05）。在调整了混杂因素的潜在影响后，通过MVMR分析，男性的雌二醇和口腔溃疡仍然没有因果关系（P=.081）。而MVMR分析显示，女性中雌二醇与口腔溃疡之间的因果关系对于少数SNP不能具有统计学意义。没有证据表明雌二醇与口腔溃疡之间存在因果关系。用雌二醇治疗口腔溃疡的策略仍需要更多的研究证实。
