PDF(Pubmed)
Abstract：
Infectious skin diseases are quite common in veterinary medicine. These diseases can be caused by both bacteria and pathogenic fungi. Antimicrobial drugs are usually used for treatment. An alternative to these drugs could be ozonated oils with antibacterial and antifungal properties. Four different ozonated oils (linseed, hemp seed, sunflower, and olive) were tested in order to develop an optimal pharmaceutical form for the treatment of skin infections in animals. Chemical parameters such as acid and acidity value, iodine and peroxide value, viscosity, and infrared spectres were analysed. The ozonation of oils resulted in changes in their chemical composition. The antimicrobial activity of the tested oils was evaluated by determining the minimum inhibitory concentrations and zones of inhibition in agar. After ozonation, the acid content increased in all the tested oils. The highest acidity was found in linseed oil (13.00 ± 0.11 mg KOH/g; 6.1%). Hemp oil, whose acidity was also significant (second only to linseed oil), was the least acidified by ozonation (11.45 ± 0.09 mg KOH/g; 5.75%). After ozonation, the iodine value in oils was significantly reduced (45-93%), and the highest amounts of iodine value remained in linseed (47.50 ± 11.94 g Iodine/100 g oil) and hemp (44.77 ± 1.41 Iodine/100 g oil) oils. The highest number of peroxides after the ozonation of oils was found in sunflower oil (382 ± 9.8 meqO2/kg). It was found that ozonated hemp and linseed oils do not solidify and remain in liquid form when the temperature drops. The results showed a tendency for the reference strains of S. aureus, E. faecalis, and E. coli to have broader zones of inhibition (p < 0.001) than clinical strains. Overall, ozonated linseed oil had the highest antibacterial activity, and ozonated olive oil had the lowest, as determined by both methods. It was found that ozonated linseed oil was the most effective on bacteria, while the most sensitive were S. aureus ATCC 25923, MRSA, and S. pseudointermedius (MIC 13.5 mg/mL, 4.6 mg/mL, and 13.5 mg/mL, respectively, and sterile zones 20.67 ± 0.98 mm, 20.25 ± 0.45 mm, and 18.25 ± 0.45 mm, respectively). The aim and new aspect of this work is the characterisation of selected ozonated vegetable oils, especially hemp oil, according to chemical and antibacterial parameters, in order to select suitable candidates for preclinical and clinical animal studies in the treatment of bacterial or fungal skin infections in terms of safety and efficacy.
摘要：
传染性皮肤病在兽医学中相当常见。这些疾病可由细菌和病原真菌引起。抗菌药物通常用于治疗。这些药物的替代品可以是具有抗菌和抗真菌特性的臭氧化油。四种不同的臭氧化油(亚麻籽，大麻种子，向日葵,和橄榄）进行了测试，以开发用于治疗动物皮肤感染的最佳药物形式。化学参数，如酸和酸度值，碘和过氧化值，粘度,并对红外光谱进行了分析。油的臭氧化导致其化学成分发生变化。通过测定在琼脂中的最小抑制浓度和抑制区评价测试油的抗微生物活性。臭氧化后,所有测试油中的酸含量增加。亚麻子油中酸度最高（13.00±0.11mgKOH/g；6.1％）。大麻油,其酸度也很高（仅次于亚麻籽油），通过臭氧化酸化最少(11.45±0.09mgKOH/g;5.75%)。臭氧化后,油中的碘值显着降低（45-93％），亚麻子（47.50±11.94g碘/100g油）和大麻（44.77±1.41碘/100g油）的碘值最高。在向日葵油中发现了油臭氧化后最高的过氧化物数量（382±9.8meqO2/kg）。发现臭氧化的大麻和亚麻籽油在温度下降时不会固化并保持液体形式。结果表明，金黄色葡萄球菌的参考菌株有一定的趋势，E.粪便，和大肠杆菌具有比临床菌株更宽的抑制区(p<0.001)。总的来说,臭氧化亚麻籽油具有最高的抗菌活性，臭氧化橄榄油含量最低,由这两种方法确定。结果发现，臭氧化亚麻籽油对细菌最有效，而最敏感的是金黄色葡萄球菌ATCC25923，MRSA，和假中间链球菌(MIC13.5mg/mL，4.6mg/mL,和13.5毫克/毫升，分别,无菌区20.67±0.98mm,20.25±0.45mm,18.25±0.45毫米，分别)。这项工作的目的和新的方面是选定的臭氧化植物油的表征，尤其是大麻油,根据化学和抗菌参数，为了在安全性和有效性方面为治疗细菌或真菌皮肤感染的临床前和临床动物研究选择合适的候选者。
