PDF(Pubmed)
Abstract：
Sheep and goats serve as crucial hosts for Cryptosporidium spp. and are primarily responsible for its transmission via the fecal-oral route. This can result in symptoms such as lamb weight loss, diarrhea, and even fatalities, leading to significant economic losses. Currently, there is a lack of scholarly research investigating the prevalence of Cryptosporidium spp. infection in sheep and goats specifically within Jiangsu province. This study collected fecal samples from sheep and goats, extracted their DNA, amplified target bands using nested PCR, sequenced the DNA, constructed a phylogenetic tree, and identified the genetic genotype. In total, 3 positive samples were identified out of 398 samples. Furthermore, the gene sequences of these samples exhibited significant homology with C. xiaoi in GenBank. The phylogenetic analysis revealed that the Cryptosporidium spp. parasites under investigation are phylogenetically related to C. xiaoi. Conducting epidemiological investigations and accurately identifying the species of Cryptosporidium spp. is of utmost importance not only for the mutton sheep farming industry in Jiangsu but also for the proactive safeguarding of human health.
摘要：
绵羊和山羊是隐孢子虫的重要宿主。并主要负责其通过粪便-口腔途径的传播。这可能会导致羔羊体重减轻等症状，腹泻,甚至死亡,造成重大经济损失。目前,缺乏研究隐孢子虫的患病率的学术研究。绵羊和山羊的感染，特别是在江苏省内。这项研究收集了绵羊和山羊的粪便样本，提取他们的DNA,使用巢式PCR扩增目标条带，测序了DNA,构建了一个系统发育树，并确定了基因型。总的来说,在398个样品中鉴定出3个阳性样品。此外，这些样品的基因序列与GenBank中的C.xiaoi表现出显著的同源性。系统发育分析表明，隐孢子虫属。被调查的寄生虫在系统发育上与C.Xiaoi有关。开展流行病学调查，准确识别隐孢子虫的种类。不仅对江苏肉羊养殖业至关重要，而且对积极维护人类健康也至关重要。
