PDF(Pubmed)
Abstract：
The oral cavity contains a vast array of microbes that contribute to the balance between oral health and disease. In addition, oral bacteria can gain access to the circulation and contribute to other diseases and chronic conditions. There are a limited number of publications available regarding the comparative lipidomics of oral bacteria and fungi involved in the construction of oral biofilms, hence our decision to study the lipidomics of representative oral bacteria and a fungus. We performed high-resolution mass spectrometric analyses (<2.0 ppm mass error) of the lipidomes from five Gram-positive commensal bacteria: Streptococcus oralis, Streptococcus intermedius, Streptococcus mitis, Streptococcus sanguinis, and Streptococcus gordonii; five Gram-positive opportunistic bacteria: Streptococcus mutans, Staphylococcus epidermis, Streptococcus acidominimus, Actinomyces viscosus, and Nanosynbacter lyticus; seven Gram-negative opportunistic bacteria: Porphyromonas gingivalis. Prevotella brevis, Proteus vulgaris, Fusobacterium nucleatum, Veillonella parvula, Treponema denticola, and Alkermansia muciniphila; and one fungus: Candida albicans. Our mass spectrometric analytical platform allowed for a detailed evaluation of the many structural modifications made by microbes for the three major lipid scaffolds: glycerol, sphingosine and fatty acyls of hydroxy fatty acids (FAHFAs).
摘要：
口腔含有大量微生物，有助于口腔健康和疾病之间的平衡。此外,口腔细菌可以进入血液循环，并导致其他疾病和慢性疾病。关于口腔生物膜构建中涉及的口腔细菌和真菌的比较脂质组学的出版物数量有限，因此，我们决定研究代表性口腔细菌和真菌的脂质组学。我们对来自五种革兰氏阳性共生细菌的脂体进行了高分辨率质谱分析（质量误差<2.0ppm）：口腔链球菌，中间链球菌,米炎链球菌，血链球菌,和格氏链球菌；五种革兰氏阳性机会致病菌：变形链球菌，表皮葡萄球菌,酸性链球菌，粘性放线菌，和纳米芽孢杆菌；七个革兰氏阴性机会细菌：牙龈卟啉单胞菌。Prevotellabrevis,普通变形杆菌,具核梭杆菌,细小静脉菌,Denticola密螺旋体,和粘蛋白Alkermansia；和一种真菌：白色念珠菌。我们的质谱分析平台可以详细评估微生物对三种主要脂质支架的许多结构修饰：甘油，羟基脂肪酸(FAHFA)的鞘氨醇和脂肪酰基。
