PDF(Pubmed)
Abstract：
Bartonella henselae is associated with numerous clinical syndromes in people. Cats are the definitive hosts for B. henselae, develop high levels of bacteremia, and are associated with human infections, particularly in the presence of Ctenocephalides felis. Several antibiotic protocols used for the treatment of B. henselae infection in cats have failed to clear bacteremia. The purpose of this study was to assess the safety and efficacy of a high-dose pradofloxacin protocol to eliminate B. henselae bacteremia. Bartonella henselae infection was initiated in 8 cats by intravenous inoculation of infected feline blood and then pradofloxacin was administered at 7.5 mg/kg, PO, twice daily for 28 days, starting 12 weeks after inoculation. Complete blood cell counts were performed prior to pradofloxacin administration and then every 2 weeks for 10 weeks. Bartonella PCR assay was performed prior to pradofloxacin administration and approximately every 2 weeks for 10 weeks and then weekly for 4 weeks. Methylprednisolone acetate (5 mg/kg) was administered by intramuscular injection to all cats on week 10. The cats remained normal and none developed a hematocrit, platelet count, lymphocyte count, or neutrophil count outside of the normal reference ranges. In the one month prior to pradofloxacin administration, all cats were PCR-positive for Bartonella DNA on at least two of four sample dates; after pradofloxacin administration, all cats were negative for B. henselae DNA in blood on all nine sample dates. The protocol appears to be safe and failure to amplify B. henselae DNA from the blood after the administration of pradofloxacin and one dose of methylprednisolone acetate suggests either an antibiotic effect or the organism was cleared spontaneously.
摘要：
汉氏巴尔通体与人的许多临床综合征有关。猫是B.henselae的确定宿主,发展高水平的菌血症，并与人类感染有关，特别是在Ctenocephalidesfelis存在的情况下。用于治疗猫中的B.henselae感染的几种抗生素方案未能清除菌血症。这项研究的目的是评估高剂量普拉多氟沙星方案消除汉森芽孢杆菌菌血症的安全性和有效性。通过静脉内接种感染的猫科动物血液，在8只猫中开始了巴尔通体感染，然后以7.5mg/kg的剂量施用了普拉氧氟沙星，PO,每天两次，共28天，接种后12周开始。在普打氧氟沙星给药前进行全血细胞计数,然后每2周进行10周。在普打氧氟沙星给药前进行巴尔通体PCR测定,大约每2周进行10周,然后每周进行4周。在第10周通过肌内注射向所有猫施用乙酸甲泼尼龙(5mg/kg)。猫保持正常，没有人出现血细胞比容，血小板计数,淋巴细胞计数,或中性粒细胞计数超出正常参考范围。在普打氧氟沙星给药前一个月，在四个样本日期中的至少两个日期，所有猫的巴尔通体DNA均为PCR阳性；在普打氧氟沙星给药后，在所有9个样本日期，所有猫的血液中的B.henselaeDNA均为阴性。该方案似乎是安全的，并且在施用普打氧氟沙星和一剂量的甲基强的松龙乙酸盐后，无法从血液中扩增汉氏芽孢杆菌DNA，这表明抗生素作用或生物体被自发清除。
