PDF(Pubmed)
Abstract：
Cattle are frequent carriers of Campylobacter spp.; therefore, these bacteria may be transmitted to humans through meat or milk. Campylobacter spp. in raw milk derives most commonly from secondary fecal contamination during the milking process; however, the udder excretion of Campylobacter may be a cause of milk-borne infection. Studies were carried out on a Campylobacter-positive farm with two different housing systems (with free-stall and tie-stall systems). The sampling process comprised several stages, including samples being taken from animals, such as from raw milk and feces, and from the environment, such as the from floor in the milking parlor and from teat cups. None of the individual raw milk samples or swabs from the floor in the parlor before the milking process were positive for Campylobacter spp. Simultaneously, Campylobacter spp. was isolated from all swabs from the floor after the milking process and in the bulk tank milk samples from the two farms. The incidence of Campylobacter isolated from fecal and teat swab samples ranged from 15.4% to 26.7% and from 8.9% to 25%, respectively. Altogether, 59 recovered Campylobacter isolates were classified, based on sequencing of the flaA short variable region, showing 15 different allele types, and the majority of them were distributed among one farm. Analysis of the virulence and antimicrobial properties showed that genes related to adherence, invasion and cytotoxicity were widely distributed among the Campylobacter recovered strains. In relation to AMR, multidrug resistance was noted in 16.1% of strains.
摘要：
牛是弯曲杆菌的频繁携带者。；因此，这些细菌可能通过肉或牛奶传播给人类。弯曲杆菌属。在原料奶中，最常见的原因是挤奶过程中的二次粪便污染；然而，乳房弯曲杆菌的排泄可能是乳源性感染的一个原因.对具有两种不同住房系统（具有自由失速和平速系统）的弯曲杆菌阳性农场进行了研究。采样过程包括几个阶段，包括从动物身上采集的样本，比如来自生牛奶和粪便，从环境来看，例如挤奶厅的地板和奶杯。挤奶过程之前，客厅地板上的单个原奶样品或棉签均未对弯曲杆菌属呈阳性。同时,弯曲杆菌属。挤奶过程后，从地板上的所有拭子中分离出来，并在两个农场的散装罐中牛奶样品中分离出来。从粪便和乳头拭子样本中分离的弯曲杆菌的发生率为15.4％至26.7％和8.9％至25％，分别。总之,对59个回收的弯曲杆菌分离株进行了分类，基于flaA短可变区的测序，显示15种不同的等位基因类型，其中大多数分布在一个农场。毒力和抗菌特性的分析表明，与粘附相关的基因，侵袭和细胞毒性在弯曲杆菌回收菌株中广泛分布。关于AMR，16.1％的菌株出现多药耐药性。
