PDF(Pubmed)
Abstract：
Ticks are an important type of pathogen transmission vector, and pathogens not only cause serious harm to livestock but can also infect humans. Because of the roles that ticks play in disease transmission, reducing tick pathogen infectivity has become increasingly important and requires the identification and characterization of these pathogens and their interaction mechanisms. In this study, we determined the miRNA expression profile of Hemaphysalis longicornis infected with Theileria orientalis, predicted the target genes of miRNAs involved in this infection process, and investigated the role of miRNA target recognition during host-pathogen interactions. The results showed that longipain is a target gene of miR-5309, which was differentially expressed at different developmental stages and in various tissues in the control group. However, the miR-5309 level was reduced in the infection group. Analysis of the interaction between miRNA and the target gene showed that miR-5309 negatively regulated the expression of the longipain protein during the infection of H. longicornis with T. orientalis. To verify this inference, we compared longipain with the blocking agent orientalis. In this study, the expression of longipain was upregulated by the inhibition of miR-5309 in ticks, and the ability of the antibody produced by the tick-derived protein to attenuate T. orientalis infection was verified through animal immunity and antigen-antibody binding tests. The results showed that expression of the longipain + GST fusion protein caused the cattle to produce antibodies that could be successfully captured by ticks, and cellular immunity was subsequently activated in the ticks, resulting in a subtractive effect on T. orientalis infection. This research provides ideas for the control of ticks and tickborne diseases and a research basis for studying the mechanism underlying the interaction between ticks and pathogens.
摘要：
蜱是一种重要的病原体传播载体，病原体不仅对牲畜造成严重危害，而且还可以感染人类。由于蜱在疾病传播中的作用，降低蜱病原体的感染性变得越来越重要，需要鉴定和表征这些病原体及其相互作用机制。在这项研究中,我们确定了被东方Theileria感染的长骨干的miRNA表达谱，预测参与该感染过程的miRNA的靶基因，并研究了miRNA靶标识别在宿主-病原体相互作用中的作用。结果表明，longipain是miR-5309的靶基因，在对照组中不同发育阶段和不同组织中差异表达。然而,感染组的miR-5309水平降低.miRNA与靶基因之间的相互作用分析表明，miR-5309在T.Orientalis感染长尾H.longicornis期间负向调节longipain蛋白的表达。为了验证这个推论，我们比较了长痛和阻断剂东方。在这项研究中,通过抑制miR-5309在蜱中的表达上调，并且通过动物免疫和抗原-抗体结合试验验证了由蜱源蛋白产生的抗体减弱东方毛虫感染的能力。结果表明，longpain+GST融合蛋白的表达导致牛产生可被蜱成功捕获的抗体，细胞免疫随后在蜱中被激活，对侧柏感染产生消减效应。本研究为蜱和蜱传疾病的控制提供了思路，为研究蜱与病原体相互作用的潜在机制提供了研究基础。
