PDF(Pubmed)
Abstract：
Recently, interest in polyphenol-containing composite adhesives for various biomedical applications has been growing. Tannic acid (TA) is a polyphenolic compound with advantageous properties, including antioxidant and antimicrobial properties. Additionally, TA contains multiple hydroxyl groups that exhibit biological activity by forming hydrogen bonds with proteins and biomacromolecules. Furthermore, TA-containing polymer composites exhibit excellent tissue adhesion properties. In this study, the gelation behavior and adhesion forces of TA/Pluronic F127 (TA/PluF) composite hydrogels were investigated by varying the TA and PluF concentrations. PluF (above 16 wt%) alone showed temperature-responsive gelation behavior because of the closely packed micelle aggregates. After the addition of a small amount of TA, the TA/PluF hydrogels showed thermosensitive behavior similar to that of PluF hydrogels. However, the TA/PluF hydrogels containing more than 10 wt% TA completely suppressed the thermo-responsive gelation kinetics of PluF, which may have been due to the hydrogen bonds between TA and PluF. In addition, TA/PluF hydrogels with 40 wt% TA showed excellent tissue adhesion properties and bursting pressure in porcine intestinal tissues. These results are expected to aid in understanding the use of mixtures of TA and thermosensitive block copolymers to fabricate adhesive hydrogels for versatile biomedical applications.
摘要：
最近,对用于各种生物医学应用的含多酚复合粘合剂的兴趣一直在增长。单宁酸(TA)是一种具有优良性能的多酚化合物,包括抗氧化和抗菌性能。此外,TA含有通过与蛋白质和生物大分子形成氢键而表现出生物活性的多个羟基。此外，含TA的聚合物复合材料表现出优异的组织粘附性能。在这项研究中,通过改变TA和PluronicF127(TA/PluF)复合水凝胶的浓度，研究了其凝胶化行为和粘附力。由于紧密堆积的胶束聚集体，单独的PluF（高于16wt％）显示出温度响应性胶凝行为。添加少量TA后，TA/PluF水凝胶表现出与PluF水凝胶相似的热敏行为。然而,含大于10wt%TA的TA/PluF水凝胶完全抑制了PluF的热响应性胶凝动力学,这可能是由于TA和PluF之间的氢键。此外,含40wt％TA的TA/PluF水凝胶在猪肠道组织中显示出优异的组织粘附性能和破裂压力。预期这些结果有助于理解使用TA和热敏嵌段共聚物的混合物来制造用于通用生物医学应用的粘合剂水凝胶。
