PDF(Pubmed)
Abstract：
Biofilm-associated infections caused by drug-resistant and persistent bacteria remain a significant clinical challenge. Here we report that farnesol, commercially available as a cosmetic and flavoring agent, shows significant anti-biofilm properties when dissolved in ethanol using a proprietary formulation emulsion technique. Farnesol in the new formulation inhibits biofilm formation and disrupts established biofilms for Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa, including their polymicrobial biofilms, and, moreover, kills S. aureus persister cells that have developed tolerance to antibiotics. No resistance to farnesol was observed for S. aureus after twenty continuous passages. Farnesol combats biofilms by direct killing, while also facilitating biofilm detachment. Furthermore, farnesol was safe and effective for preventing and treating biofilm-associated infections of both types of bacteria in an ex vivo burned human skin model. These data suggest that farnesol in the new formulation is an effective broad-spectrum anti-biofilm agent with promising clinical potential. Due to its established safety, low-cost, versatility, and excellent efficacy-including ability to reduce persistent and resistant microbial populations-farnesol in the proprietary formulation represents a compelling transformative, translational, and commercial platform for addressing many unsolved clinical challenges.
摘要：
由耐药性和持久性细菌引起的生物膜相关感染仍然是一个重大的临床挑战。在这里我们报告法尼醇，市售作为化妆品和调味剂，当使用专有的制剂乳液技术溶解在乙醇中时,显示出显著的抗生物膜性质。新制剂中的法尼醇抑制生物膜形成并破坏革兰氏阳性金黄色葡萄球菌和革兰氏阴性铜绿假单胞菌的生物膜，包括它们的多微生物生物膜，and,此外,杀死对抗生素产生耐受性的金黄色葡萄球菌耐受细胞。在连续二十次传代后,对于金黄色葡萄球菌没有观察到对法尼醇的抗性。法尼醇通过直接杀死来对抗生物膜，同时也促进生物膜的脱离。此外，法尼醇对于预防和治疗离体烧伤人类皮肤模型中两种类型细菌的生物膜相关感染是安全和有效的。这些数据表明，新制剂中的法尼醇是一种有效的广谱抗生物膜剂，具有良好的临床潜力。由于其既定的安全性，低成本,多功能性，和出色的功效-包括减少持久性和耐药性微生物种群的能力-法尼醇在专有配方中代表了引人注目的变革，翻译,以及解决许多未解决的临床挑战的商业平台。
