PDF(Pubmed)
Abstract：
Representatives of the genus Sarcocystis are worldwide distributed apicomplexan parasites characterised by two-host prey-predator relationships. Sarcocystis spp. produce sarcocysts in the muscles and brains of intermediate hosts and develop sporocysts in the intestines of definitive hosts. Two species, Sarcocystis glareoli and Sarcocystis microti, previously assigned to the genus Frenkelia, form cysts in the brains of rodents and are transmitted through the common buzzard (Buteo buteo). In our study, brain samples of 694 small mammals caught in different regions of Lithuania were examined for Sarcocystis spp. Additionally, 10 B. buteo and two rough-legged buzzards (Buteo lagopus) were tested for sporocysts of the analysed parasites. Sarcocystis species were identified based on 28S rRNA sequence comparison. Of the eleven species of small mammals tested, Sarcocystis parasites were observed only in the bank vole (Clethrionomys glareolus). Cysts of S. glareoli were detected in 34 out of 374 C. glareolus (9.1%, 95% CI = 6.4-12.5%). Molecular investigation showed the presence of only S. glareoli in the intestines of 50% of B. buteo. Furthermore, two species, Sarcocystis sp. Rod3 and Sarcocystis sp. Rod4, were confirmed in B. lagopus. Our results demonstrate the need for further studies on Sarcocystis cycling between rodents and birds.
摘要：
Sarcocystis属的代表是全球分布的顶plex寄生虫，其特征是两个宿主的猎物-捕食者关系。结节虫。在中间宿主的肌肉和大脑中产生肌囊，并在确定宿主的肠道中产生孢子囊。两个物种,glareoli和microSarcocystis，以前分配给Frenkelia属，在啮齿动物的大脑中形成囊肿，并通过普通秃鹰（Buteobuteo）传播。在我们的研究中,检查了在立陶宛不同地区捕获的694只小型哺乳动物的脑样本中的肉囊虫。此外,测试了B.buteo和两个粗腿秃鹰（Buteolagopus）的被分析寄生虫的孢子囊。基于28SrRNA序列比较鉴定了结节虫物种。在测试的11种小型哺乳动物中，仅在田鼠（Clethrionomysglareolus）中观察到了结节虫寄生虫。在374个C.glareolus中的34个中检测到了S.glareoli的囊肿（9.1％，95%CI=6.4-12.5%）。分子研究表明，在肠道中只有50％的B.buteo仅存在S.glareoli。此外，两个物种,结节虫sp.Rod3andSarcocystissp.Rod4在B.lagopus中被证实。我们的结果表明，需要进一步研究啮齿动物和鸟类之间的肉囊虫循环。
