UNASSIGNED: We examined whether addition of IL-2 restores the Treg proportion even with concurrent use of a calcineurin inhibitor and if the follicular helper T cell (Tfh) proportion is reduced in an SLE-like murine chronic graft versus host disease model.
UNASSIGNED: Using a parent-into-F1 model, we investigated the effect of IL-2 plus tacrolimus on Treg and Tfh proportions and the therapeutic effect.
UNASSIGNED: Treatment with a combination of IL-2 and tacrolimus significantly delayed the initiation of proteinuria and decreased the urinary protein concentration, whereas tacrolimus or IL-2 monotherapy did not significantly attenuate proteinuria. Phosphorylation of signal transducer and activator of transcription 3, a positive regulator of Tfh differentiation, was reduced by combination treatment, whereas phosphorylation of signal transducer and activator of transcription 5, a negative regulator, was not reduced.
UNASSIGNED: Addition of calcineurin inhibitors as adjunct agents may be beneficial for IL-2-based treatment of lupus nephritis.
■我们研究了在SLE样小鼠慢性移植物抗宿主疾病模型中,即使同时使用钙调磷酸酶抑制剂,IL-2的添加是否恢复了Treg比例,以及滤泡辅助性T细胞(Tfh)比例是否降低。
■使用父入F1模型,我们研究了IL-2联合他克莫司对Treg和Tfh比例的影响以及治疗效果。
■用IL-2和他克莫司联合治疗显著延缓了蛋白尿的开始,降低了尿蛋白浓度,而他克莫司或IL-2单药治疗未显著减轻蛋白尿。信号转导和转录激活因子3的磷酸化,Tfh分化的正调节因子,通过联合治疗减少了,而信号转导和转录激活因子5的磷酸化,负调节因子,没有减少。
■添加钙调磷酸酶抑制剂作为辅助药物可能有益于基于IL-2的狼疮性肾炎的治疗。