Abstract：
Toxocara canis can produce the \"larva migrans\" syndrome in humans, and in puppies, it can cause severe digestive disorders. The most used treatments are based on anthelmintics, although there are reports of anthelmintic (AH) resistance. The Yucatan Peninsula has a great variety of plant species whose AH properties are still unknown. The objective of this study was to evaluate the in vitro AH activity of ethanolic (EE), methanolic (ME) and aqueous (AE) extracts from the leaves of five native plant species of the Yucatan Peninsula on T. canis eggs of dogs from Merida, Yucatan. As part of a screening, the EE of the plants Alseis yucatanensis, Calea jamaicensis, Cameraria latifolia, Macrocepis diademata, and Parathesis cubana were evaluated at doses of 2400 and 3600 μg/ml. The EE and AE of A. yucatanensis and M. diademata presented high percentages (≥ 91.3%) of inhibition of the larval development of T. canis after six days of exposure. The lowest LC50 and LC99 was presented by the ME from A. yucatanensis (255.5 and 629.06 µg/ml, respectively) and the ME from M. diademata (222.4 and 636.5 µg/ml, respectively), and the AE from A. yucatanenesis (LC50 of 535.9 µg/ml). Chemical profiling of the most potent AH extract (Alseis yucatanensis) was carried out by LC-UV-HRMS. Data from the ME and AE from this plant indicated the presence of the known glucosylngoumiensine, kaempferol 3,7-diglucosyde, uvaol, linoleic acid and linolenic acid together with unknown alkaloids. The EE, ME and AE from leaves of M. diademata and A. yucatanensis could be developed as natural alternatives to control T. canis.
摘要：
犬弓形虫会在人类中产生“幼虫偏头痛”综合征，在小狗身上，它会导致严重的消化系统疾病。最常用的治疗方法是基于驱虫药，尽管有驱虫药（AH）耐药的报道。尤卡坦半岛有各种各样的植物物种，其AH特性仍然未知。本研究的目的是评估乙醇（EE）的体外AH活性，来自梅里达犬的T.canis卵的尤卡坦半岛五种本地植物物种的叶子中的甲醇（ME）和水性（AE）提取物，尤卡坦半岛.作为筛选的一部分,Alseisyucatanensis植物的EE，牙买加Calea,金花,双色马，并在2400和3600μg/ml的剂量下评估了古巴的寄生虫。Yucatanensis和M.diademata的EE和AE在暴露六天后对T.canis幼虫发育的抑制作用很高（≥91.3％）。最低的LC50和LC99由来自Yucatanensis的ME呈现（255.5和629.06µg/ml，分别）和来自M.diademata的ME（222.4和636.5µg/ml，分别)，和来自A.yucatanenesis的AE（LC50为535.9µg/ml）。通过LC-UV-HRMS对最有效的AH提取物（Alseisyucatanensis）进行了化学分析。来自该植物的ME和AE的数据表明存在已知的葡萄糖基杜美辛，山奈酚3,7-二葡萄糖基，uvaol,亚油酸和亚麻酸以及未知的生物碱。EE,可以开发来自M.diademata和A.yucatanensis叶片的ME和AE作为控制T.canis的天然替代品。
