Abstract：
Scorpion fluorescence under ultraviolet light is a well-known phenomenon, but its features under excitation in the UVA, UVB and UVC bands have not been characterized. Systematic fluorescence characterization revealed indistinguishable fluorescence spectra with a peak wavelength of 475 nm for whole exuviae from second-, third- and fifth-instar scorpions under different ultraviolet light ranges. In-depth investigations of the chelae, mesosoma, metasoma and telson of adult scorpions further indicated heterogeneity in the typical fluorescence spectrum within the visible light range and in the newly reported fluorescence spectrum with a peak wavelength of 320 nm within the ultraviolet light range, which both showed excitation wavelength-independent features. Dynamic fluorescence changes during the molting process of third-instar scorpions revealed the fluorescence heterogeneity-dependent recovery speed of scorpion exoskeletons. The typical fluorescence spectra of the molted chelae and telson rapidly recovered approximately 6 h after ecdysis under UVA light and approximately 36 h after ecdysis under UVB and UVC light. However, it took approximately 12 h and 24 h to obtain the typical fluorescence spectra of the molted metasoma and mesosoma, respectively, under UVA irradiation and 72 h to obtain the typical fluorescence spectra under UVB and UVC irradiation. The fluorescence heterogeneity-dependent fluorescence recovery of the scorpion exoskeleton was further confirmed by tissue section analysis of different segments from molting third-instar scorpions. These findings reveal novel scorpion fluorescence features and provide potential clues on the biological function of scorpion fluorescence.
摘要：
紫外线下的蝎子荧光是众所周知的现象，但是它在UVA激励下的特征，UVB和UVC频带尚未被表征。系统的荧光表征揭示了无法区分的荧光光谱，其峰值波长为475nm，在不同的紫外线范围下，三龄和五龄蝎子。对龟甲的深入研究，间质瘤,成年蝎子的转移瘤和telson进一步表明，在可见光范围内的典型荧光光谱和在紫外线范围内的峰值波长为320nm的新报道的荧光光谱中存在异质性，两者都显示出与激发波长无关的特征。三龄蝎子蜕皮过程中的动态荧光变化揭示了蝎子外骨骼的荧光异质性依赖性恢复速度。蜕皮和telson的典型荧光光谱在UVA光下蜕皮后约6小时，在UVB和UVC光下蜕皮后约36小时迅速恢复。然而,大约需要12小时和24小时才能获得蜕变转移瘤和中膜瘤的典型荧光光谱，分别,在UVA照射和72h下获得UVB和UVC照射下的典型荧光光谱。通过对蜕皮三龄蝎子的不同节段的组织切片分析，进一步证实了蝎子外骨骼的荧光异质性依赖性荧光恢复。这些发现揭示了蝎子荧光的新特征，并为蝎子荧光的生物学功能提供了潜在的线索。
