PDF(Pubmed)
Abstract：
OBJECTIVE: The aim of this study was to identify Echinococcus species by morphological and molecular means.
METHODS: A dead gray wolf (Canis lupus) was found near Erzurum province and brought to the parasitology laboratory. Sedimentation and counting technique (SCT) and polymerase chain reaction (PCR) analysis were conducted.
RESULTS: The SCT implications indicated that the wolf had a substantial worm burden (62,720 and 49,280 parasites) due to a co-infection of E. granulosus s.l. and E. multilocularis. Genus/species-specific PCR was used to analyze DNA extracted from adult worms and confirmed as E. granulosus s.s. and E. multilocularis, utilizing COI and 12S rRNA gene sequence analysis, respectively.
CONCLUSIONS: This report presents the first co-detection of E. granulosus s.s. and E. multilocularis in a gray wolf found in an urban area in a highly endemic area for human echinococcosis in northeastern Turkey. The results emphasize that AE is not only a problem of rural areas, but also occurs in urban areas, which may pose a threat to public health. Therefore, surveillance in urban areas is crucial. The need to develop new control strategies for domestic and wildlife in the study area is also highlighted.
METHODS: A dead gray wolf (Canis lupus) was found near Erzurum province and brought to the parasitology laboratory. Sedimentation and counting technique (SCT) and polymerase chain reaction (PCR) analysis were conducted.
RESULTS: The SCT implications indicated that the wolf had a substantial worm burden (62,720 and 49,280 parasites) due to a co-infection of E. granulosus s.l. and E. multilocularis. Genus/species-specific PCR was used to analyze DNA extracted from adult worms and confirmed as E. granulosus s.s. and E. multilocularis, utilizing COI and 12S rRNA gene sequence analysis, respectively.
CONCLUSIONS: This report presents the first co-detection of E. granulosus s.s. and E. multilocularis in a gray wolf found in an urban area in a highly endemic area for human echinococcosis in northeastern Turkey. The results emphasize that AE is not only a problem of rural areas, but also occurs in urban areas, which may pose a threat to public health. Therefore, surveillance in urban areas is crucial. The need to develop new control strategies for domestic and wildlife in the study area is also highlighted.
摘要：
目的：本研究的目的是通过形态学和分子手段鉴定棘球蚴的种类。
方法：在埃尔祖鲁姆省附近发现了一只死灰狼（犬狼疮），并将其带到寄生虫学实验室。进行沉降和计数技术（SCT）和聚合酶链反应（PCR）分析。
结果：SCT的含义表明，由于同时感染了E.granulosuss.l。和多房性大肠杆菌，狼具有大量的蠕虫负担（62,720和49,280寄生虫）。属/种特异性PCR用于分析从成虫中提取的DNA，并确认为E.granulosuss.s.和E.利用COI和12SrRNA基因序列分析，分别。
结论：本报告首次共同检测了在土耳其东北部人类包虫病高度流行区的城市地区发现的灰狼中的E.granulosuss.s.和E.multilocularis。结果强调，AE不仅是农村地区的问题，但也发生在城市地区，这可能对公众健康构成威胁。因此,城市地区的监控至关重要。还强调了需要为研究区域的家庭和野生动植物开发新的控制策略。
方法：在埃尔祖鲁姆省附近发现了一只死灰狼（犬狼疮），并将其带到寄生虫学实验室。进行沉降和计数技术（SCT）和聚合酶链反应（PCR）分析。
结果：SCT的含义表明，由于同时感染了E.granulosuss.l。和多房性大肠杆菌，狼具有大量的蠕虫负担（62,720和49,280寄生虫）。属/种特异性PCR用于分析从成虫中提取的DNA，并确认为E.granulosuss.s.和E.利用COI和12SrRNA基因序列分析，分别。
结论：本报告首次共同检测了在土耳其东北部人类包虫病高度流行区的城市地区发现的灰狼中的E.granulosuss.s.和E.multilocularis。结果强调，AE不仅是农村地区的问题，但也发生在城市地区，这可能对公众健康构成威胁。因此,城市地区的监控至关重要。还强调了需要为研究区域的家庭和野生动植物开发新的控制策略。
