PDF(Pubmed)
Abstract：
UNASSIGNED: The diagnosis of Precancerous Lesions of Gastric Cancer (PLGC) is challenging in clinical practice. We conducted a clinical study by analyzing the information of relevant chromosome copy number variations (CNV) in the TCGA database followed by the UCAD technique to evaluate the value of Chromosomal Instability (CIN) assay in the diagnosis of PLGC.
UNASSIGNED: Based on the screening of gastric cancer related data in TCGA database, CNV analysis was performed to explore the information of chromosome CNV related to gastric cancer. Based on the gastroscopic pathology results, 12 specimens of patients with severe atrophy were screened to analyze the paraffin specimens of gastric mucosa by UCAD technology, and to explore the influence of related factors on them.
UNASSIGNED: The results of CNV in TCGA database suggested that chromosome 7, 8, and 17 amplification was obvious in patients with gastric cancer. UCAD results confirmed that in 12 patients with pathologic diagnosis of severe atrophy, five of them had positive results of CIN, with a positive detection rate of 41.7%, which was mainly manifested in chromosome seven and chromosome eight segments amplification. We also found that intestinalization and HP infection were less associated with CIN. And the sensitivity of CIN measurement results was significantly better than that of tumor indicators.
UNASSIGNED: The findings suggest that the diagnosis of PLGC can be aided by UCAD detection of CIN, of which Chr7 and 8 may be closely related to PLGC.
UNASSIGNED: Based on the screening of gastric cancer related data in TCGA database, CNV analysis was performed to explore the information of chromosome CNV related to gastric cancer. Based on the gastroscopic pathology results, 12 specimens of patients with severe atrophy were screened to analyze the paraffin specimens of gastric mucosa by UCAD technology, and to explore the influence of related factors on them.
UNASSIGNED: The results of CNV in TCGA database suggested that chromosome 7, 8, and 17 amplification was obvious in patients with gastric cancer. UCAD results confirmed that in 12 patients with pathologic diagnosis of severe atrophy, five of them had positive results of CIN, with a positive detection rate of 41.7%, which was mainly manifested in chromosome seven and chromosome eight segments amplification. We also found that intestinalization and HP infection were less associated with CIN. And the sensitivity of CIN measurement results was significantly better than that of tumor indicators.
UNASSIGNED: The findings suggest that the diagnosis of PLGC can be aided by UCAD detection of CIN, of which Chr7 and 8 may be closely related to PLGC.
摘要：
■胃癌前病变（PLGC）的诊断在临床实践中具有挑战性。我们通过分析TCGA数据库中相关染色体拷贝数变异（CNV）的信息，然后使用UCAD技术进行临床研究，以评估染色体不稳定（CIN）测定在PLGC诊断中的价值。
■基于TCGA数据库中胃癌相关数据的筛选，进行CNV分析以探索与胃癌相关的染色体CNV信息。根据胃镜病理结果,用UCAD技术筛选12例重度萎缩患者的胃黏膜石蜡标本,并探讨相关因素对其的影响。
■TCGA数据库中的CNV结果表明，胃癌患者的7、8和17号染色体扩增明显。UCAD结果证实，在12例病理诊断为严重萎缩的患者中，其中五个有CIN阳性结果，阳性检出率为41.7%，主要表现在染色体7段和染色体8段扩增。我们还发现肠化和HP感染与CIN的相关性较低。CIN测量结果的敏感性明显优于肿瘤指标。
■研究结果表明，可以通过UCAD对CIN的检测来辅助PLGC的诊断，其中Chr7和Chr8可能与PLGC密切相关。
■基于TCGA数据库中胃癌相关数据的筛选，进行CNV分析以探索与胃癌相关的染色体CNV信息。根据胃镜病理结果,用UCAD技术筛选12例重度萎缩患者的胃黏膜石蜡标本,并探讨相关因素对其的影响。
■TCGA数据库中的CNV结果表明，胃癌患者的7、8和17号染色体扩增明显。UCAD结果证实，在12例病理诊断为严重萎缩的患者中，其中五个有CIN阳性结果，阳性检出率为41.7%，主要表现在染色体7段和染色体8段扩增。我们还发现肠化和HP感染与CIN的相关性较低。CIN测量结果的敏感性明显优于肿瘤指标。
■研究结果表明，可以通过UCAD对CIN的检测来辅助PLGC的诊断，其中Chr7和Chr8可能与PLGC密切相关。
