PDF(Pubmed)
Abstract：
Gene expression for Th1/Th2 cytokines (IL-4 and IFN-ɣ), regulatory cytokines (TGF-β and IL-10) and the transcriptional factor FoxP3 was analyzed in the liver and hepatic lymph nodes (HLN) from sheep immunized with partially protective and non-protective vaccine candidates and challenged with Fasciola hepatica. FoxP3 T cells were also evaluated by immunohistochemistry (IHQ). The most remarkable difference between the partially protected vaccinated (V1) group and the non-protected vaccinated (V2) group was a more severe expansion of FoxP3 T cells recorded by IHQ in both the liver and HLN of the V2 group as compared to the V1 group, whereas no differences were found between the V2 group and the infected control (IC) group. Similar results were recorded for FoxP3 gene expression although significant differences among V1 and V2 groups were only significant in the HLN, while FoxP3 gene expression was very similar in the V2 and IC groups both in the liver and HLN. No significant differences for the remaining cytokines were recorded between the V1 and V2 groups, but in the liver the V2 group shows significant increases of IFN-ɣ and IL-10 as compared to the uninfected control (UC) group whereas the V1 group did not. The lower expansion of FoxP3 T cells and lower increase of IFN-ɣ and IL-10 in the partially protected vaccinated group may be related with lower hepatic lesions and fluke burdens recorded in this group as compared to the other two infected groups. The most relevant change in regulatory cytokine gene expression was the significant increase of TGF-β in the liver of IC, V1 and V2 groups as compared to the UC group, which could be related to hepatic lesions.
摘要：
Th1/Th2细胞因子的基因表达（IL-4和IFN-），在用部分保护性和非保护性候选疫苗免疫并用肝片吸虫攻击的绵羊的肝脏和肝淋巴结（HLN）中分析了调节性细胞因子（TGF-β和IL-10）和转录因子FoxP3。还通过免疫组织化学(IHQ)评估了FoxP3T细胞。部分受保护的接种疫苗（V1）组与未受保护的接种疫苗（V2）组之间的最显著差异是，与V1组相比，IHQ记录的FoxP3T细胞在V2组的肝脏和HLN中的扩增更严重。而V2组和感染对照（IC）组之间没有发现差异。FoxP3基因表达的结果相似，尽管V1和V2组之间的显着差异仅在HLN中显著，而FoxP3基因表达在肝脏和HLN的V2和IC组中非常相似。其余细胞因子在V1和V2组之间没有显著差异。但在肝脏中，与未感染的对照（UC）组相比，V2组的IFN-和IL-10显着增加，而V1组则没有。与其他两个感染组相比，部分受保护的疫苗接种组中FoxP3T细胞的较低扩增和IFN-a和IL-10的较低增加可能与该组中记录的较低肝损伤和吸虫负担有关。调节细胞因子基因表达的最相关变化是IC肝脏中TGF-β的显着增加，V1和V2组与UC组相比，可能与肝脏病变有关.
