PDF(Pubmed)
Abstract：
Toxoplasma gondii and Neospora caninum are two closely related protozoans that infect a wide range of animals, including birds. However, the occurrence of N. caninum and T. gondii in seabirds is unknown. Therefore, this study aimed to determine the presence of T. gondii and N. caninum DNA in tissue samples of seabirds. Tissue samples of the pectoral muscles, heart, and brain were collected from 47 birds along the coastline of Santa Catarina State, SC, Brazil. The DNA was extracted from the tissues and screened using nested-PCR (nPCR) targeting internal transcribed spacer 1 (ITS1). T. gondii DNA was detected in tissues from seven seabirds (7/47, 14.8%), kelp gull (Larus dominicanus) (5/21), and Manx shearwater (Puffinus puffinus) (2/8). N. caninum DNA was detected in tissues of nine seabirds (9/47, 19.1%), the kelp gull (L. dominicanus) (4/21), Manx shearwater (P. puffinus) (2/8), neotropic cormorant (Phalacrocorax brasilianus) (1/4), brown booby (Sula leucogaster) (1/5), and white-chinned petrel (Procellaria aequinoctialis) (1/1); however, no co-infection was observed. In conclusion, this study showed the circulation of N. caninum and T. gondii in seabirds along the coastline of Santa Catarina State. Further studies are required to clarify the role of these birds in the epidemiology of neosporosis and toxoplasmosis.
摘要：
弓形虫和犬新孢子虫是两种密切相关的原生动物，感染范围广泛，包括鸟类。然而,海鸟中是否存在犬只和弓形虫。因此,本研究旨在确定海鸟组织样本中弓形虫和犬奈瑟菌DNA的存在。胸肌的组织样本,心,大脑是从圣卡塔琳娜州海岸线上的47只鸟类中收集的，SC,巴西。从组织中提取DNA并使用巢式PCR(nPCR)靶向内部转录间隔区1(ITS1)进行筛选。在7只海鸟的组织中检测到弓形虫DNA（7/47，14.8％），海带海鸥（Larusdominicanus）（5/21），和马克斯剪水（海雀）（2/8）。在9只海鸟的组织中检测到犬N.caninumDNA（9/47，19.1％），海带海鸥（L.dominicanus)(4/21),Manxshearwater(P.海雀)(2/8),新性cor（巴西鹿茸）（1/4），棕色布比（苏拉leucogaster）（1/5），和白色的海燕（Procellariaaequinoctialis）（1/1）；但是，未观察到共感染。总之,这项研究显示了圣卡塔琳娜州海岸线上海鸟中的犬奈瑟菌和弓形虫的循环。需要进一步的研究来阐明这些鸟类在新孢子虫病和弓形虫病流行病学中的作用。
