PDF(Pubmed)
Abstract：
Campylobacter jejuni (C. jejuni) is a common pathogen that often causes diarrhea, loss of appetite, and even enteritis in domestic cats, affecting their growth and development, especially in kittens under 6 months of age. Oral passive immunization with chicken yolk antibody Y has been proved effective for the treatment of gastrointestinal pathogen infections due to its high specificity. In this study, C. jejuni was isolated from diarrheal cat feces, and the specific egg yolk antibody Y against C. jejuni was demonstrated to effectively inhibit its proliferation in vitro experiments. To evaluate the effect of anti-C. jejuni IgY, the mouse C. jejuni infection model was established and it was found that IgY could alleviate C. jejuni-induced clinical symptoms. Consistent with these results, the reduction of pro-inflammatory factors and intestinal colonization by C. jejuni in the IgY-treated groups, especially in the high dose group. We then evaluated the protective effect of IgY on young Ragdoll cats infected with C. jejuni. This specific antibody reduced the rate of feline diarrhea, protected the growth of young cats, inhibited systemic inflammatory hyperactivation, and increased fecal short-chain fatty acid concentrations. Notably, IgY may have a protective role by changing intestinal amino acid metabolism and affecting C. jejuni chemotaxis. Collectively, specific IgY is a promising therapeutic strategy for C. jejuni-induced cat diarrhea.
摘要：
空肠弯曲杆菌（C.空肠)是一种常见的病原体，通常会导致腹泻，食欲不振,甚至家猫的肠炎，影响他们的成长和发展，尤其是6个月以下的小猫。用鸡卵黄抗体Y口服被动免疫由于其高特异性而被证明可有效治疗胃肠道病原体感染。在这项研究中,从腹泻猫粪便中分离出空肠杆菌，体外实验证明，针对空肠弯曲菌的特异性卵黄抗体Y能有效抑制其增殖。评价抗C.空肠IgY,建立小鼠空肠杆菌感染模型,发现IgY能缓解空肠杆菌引起的临床症状。与这些结果一致，在IgY治疗组中，空肠弯曲杆菌减少促炎因子和肠道定植，尤其是高剂量组。然后我们评估了IgY对感染空肠弯曲杆菌的幼小布娃娃猫的保护作用。这种特异性抗体降低了猫腹泻的发生率，保护幼猫的成长,抑制全身炎症过度激活，粪便短链脂肪酸浓度增加。值得注意的是,IgY可能通过改变肠道氨基酸代谢和影响空肠弯曲杆菌趋化性而具有保护作用。总的来说，特异性IgY是空肠弯曲菌引起的猫腹泻的有希望的治疗策略。
