PDF(Pubmed)
Abstract：
Sepsis is a systemic inflammatory response syndrome that develops in the host against microorganisms. This response develops away from the primary infection site and results in end-organ damage. The present study aimed to investigate the protective and therapeutic effects on lung and kidney tissue of silymarin (S) and dexmedetomidine (DEX) applied 1 h before and after sepsis induced by the cecal ligation and puncture (CLP) method in rats. A total of 62 rats was randomly divided into eight groups: i) Control (n=6); ii) cecal perforation (CLP; n=8); iii) S + CLP (n=8; S + CLP; S administered 1 h before CPL); iv) CLP + S (n=8; S administered 1 h after CLP); v) DEX + CLP (n=8; D + CLP; DEX administered 1 h before CLP); vi) CLP + D (n=8; DEX administered 1 h after CLP); vii) SD + CLP (n=8; S and DEX administered 1 h before CLP) and viii) CLP + SD (n=8; S and DEX administered 1 h after CLP). After the cecum filled with stool, it was tied with 3/0 silk under the ileocecal valve and the anterior surface of the cecum was punctured twice with an 18-gauge needle. A total of 100 mg/kg silymarin and 100 µg/kg DEX were administered intraperitoneally to the treatment groups. Lung and kidney tissue samples were collected to evaluate biochemical and histopathological parameters. In the histopathological examination, all parameters indicating kidney injury; interstitial edema, peritubular capillary dilatation, vacuolization, ablation of tubular epithelium from the basement membrane, loss of brush border in the proximal tubule epithelium, cell swelling and nuclear defragmentation; were increased in the CLP compared with the control group. Silymarin administration increased kidney damage, including ablation of tubular epithelium from the basement membrane, compared with that in the CLP group. DEX significantly reduced kidney damage compared with the CLP and silymarin groups. The co-administration of DEX + silymarin decreased kidney damage, although it was not as effective as DEX-alone. To conclude, intraperitoneal DEX ameliorated injury in CLP rats. DEX + silymarin partially ameliorated injury but silymarin administration increased damage. As a result, silymarin has a negative effects with this dosage and DEX has a protective effect. In the present study, it was determined that using the two drugs together had a greater therapeutic effect than silymarin and no differences in the effects were not observed any when the application times of the agents were changed.
摘要：
脓毒症是在宿主中针对微生物发展的全身性炎症反应综合征。这种反应远离原发感染部位发展并导致终末器官损伤。本研究旨在探讨盲肠结扎穿孔法(CLP)诱导脓毒症前后1h水飞蓟素(S)和右美托咪定(DEX)对大鼠肺、肾组织的保护作用。将总共62只大鼠随机分为八组：i）对照组（n=6）；ii）盲肠穿孔（CLP；n=8）；iii）SCLP（n=8；SCLP；CPL前1小时给药S）；iv）CLPS（n=8；CLP后1小时给药S）；v）DEXCLP（n=CLP1后8；DEPviCLP后8盲肠充满大便后，用3/0丝绑在回盲瓣下，用18号针穿刺盲肠前表面两次。总共100mg/kg水飞蓟素和100μg/kgDEX腹膜内给予治疗组。收集肺和肾组织样品以评估生化和组织病理学参数。在组织病理学检查中，所有表明肾损伤的参数；间质性水肿，肾小管周围毛细血管扩张,真空化,从基底膜消融肾小管上皮，近端小管上皮刷状缘缺失，细胞肿胀和核碎片整理；与对照组相比，CLP增加。水飞蓟素给药增加肾损伤，包括从基底膜消融肾小管上皮，与CLP组相比。与CLP和水飞蓟素组相比，DEX可显着减轻肾脏损害。DEX+水飞蓟素的共同给药减少了肾脏损伤，虽然它不如DEX单独有效。最后，腹腔DEX改善CLP大鼠损伤。DEX水飞蓟素部分改善损伤，但水飞蓟素给药增加损伤。因此,水飞蓟素具有此剂量的负面影响，DEX具有保护作用。在本研究中,已确定，与水飞蓟素相比，将两种药物一起使用具有更大的治疗效果，并且当药物的使用时间改变时，没有观察到任何效果差异。
