Abstract：
BACKGROUND: The advances of self-crossing hyaluronic acid (SC-HA) fillers combination use with polydioxanone thread in minipigs were examined for compatibility, effectiveness, and immune response.
METHODS: A 12-week experiment was conducted using 6 minipigs (3 male and 3 female each) to evaluate the effects of SC-HA filler. The molecular weight of SC-HA filler was fixed at 200 kDa and alternative storage modulus of G80, G250, and G500 were examined. The procedure involved injecting SC-HA filler and polydioxanone threads into the skin tissue of anesthetized minipigs, and tissue sampling after 1 month (three minipigs), and 3 months (three minipigs) for histological staining and analysis. The immune reaction was observed during the experiment.
RESULTS: The practitioner reported it was easy to inject the SC-HA filler in combination with polydioxanone threads. All four storage modulus of SC-HA fillers were injectable within the polydioxanone thread containing cannula. Also, during the procedure, there were no immune responses at the treated sites. The results of the histological tissue examination confirmed that there was no chemical interaction between SC-HA filler and the existing polydioxanone thread, and it was observed that SC-HA filler was more uniformly distributed within the tissue with lower storage modulus, resulting in a higher production of collagen in the surrounding filler. When combined with scaffold polydioxanone thread, the scaffold polydioxanone thread helped spread the filler evenly, resulting in a more evenly distributed collagen around the filler.
CONCLUSIONS: Today, the combination therapy of filler and polydioxanone thread in one procedure is challenging due to the high viscosity of conventional fillers. However, this study confirmed that combination therapy of filler and polydioxanone thread is possible with SC-HA fillers. Additionally, it was found that polydioxanone thread does not seem to interfere with the crosslinking reaction of SC-HA filler, and if used with a higher pH of polydioxanone, it may enhance the cross-linking reaction and achieve a higher viscosity value. Finally, the study resulted in the idea of concrete as SC-HA filler and reinforcing rod for polydioxanone thread.
METHODS: A 12-week experiment was conducted using 6 minipigs (3 male and 3 female each) to evaluate the effects of SC-HA filler. The molecular weight of SC-HA filler was fixed at 200 kDa and alternative storage modulus of G80, G250, and G500 were examined. The procedure involved injecting SC-HA filler and polydioxanone threads into the skin tissue of anesthetized minipigs, and tissue sampling after 1 month (three minipigs), and 3 months (three minipigs) for histological staining and analysis. The immune reaction was observed during the experiment.
RESULTS: The practitioner reported it was easy to inject the SC-HA filler in combination with polydioxanone threads. All four storage modulus of SC-HA fillers were injectable within the polydioxanone thread containing cannula. Also, during the procedure, there were no immune responses at the treated sites. The results of the histological tissue examination confirmed that there was no chemical interaction between SC-HA filler and the existing polydioxanone thread, and it was observed that SC-HA filler was more uniformly distributed within the tissue with lower storage modulus, resulting in a higher production of collagen in the surrounding filler. When combined with scaffold polydioxanone thread, the scaffold polydioxanone thread helped spread the filler evenly, resulting in a more evenly distributed collagen around the filler.
CONCLUSIONS: Today, the combination therapy of filler and polydioxanone thread in one procedure is challenging due to the high viscosity of conventional fillers. However, this study confirmed that combination therapy of filler and polydioxanone thread is possible with SC-HA fillers. Additionally, it was found that polydioxanone thread does not seem to interfere with the crosslinking reaction of SC-HA filler, and if used with a higher pH of polydioxanone, it may enhance the cross-linking reaction and achieve a higher viscosity value. Finally, the study resulted in the idea of concrete as SC-HA filler and reinforcing rod for polydioxanone thread.
摘要：
背景：对小型猪中自交透明质酸（SC-HA）填充剂与聚二恶烷酮线组合使用的相容性进行了检查，有效性,和免疫反应。
方法：使用6只小型猪（各3只雄性和3只雌性）进行了12周的实验，以评估SC-HA填充剂的作用。SC-HA填料的分子量固定在200kDa,并且检测G80、G250和G500的替代储能模量。该程序包括将SC-HA填充剂和聚二恶烷酮线注射到麻醉的小型猪的皮肤组织中，和组织取样后1个月（三只小型猪），和3个月(三只小型猪)进行组织学染色和分析。实验过程中观察到免疫反应。
结果：从业者报告说，将SC-HA填充剂与聚二恶烷酮线结合注射很容易。SC-HA填料的所有四种储能模量均可在含有聚二恶烷酮螺纹的套管内注射。此外，在手术过程中,在治疗部位没有免疫反应.组织学组织检查结果证实，SC-HA填料与现有聚二恶烷酮线之间没有化学相互作用，并且观察到SC-HA填料更均匀地分布在具有较低储能模量的组织内，导致周围填充物中胶原蛋白的产量更高。当与支架聚二恶烷酮线结合时，支架聚二恶烷酮线帮助填料均匀铺开，导致填充剂周围更均匀分布的胶原蛋白。
结论：今天，由于常规填充剂的高粘度，在一个程序中填充剂和聚二恶烷酮线的联合治疗具有挑战性。然而,这项研究证实，使用SC-HA填充剂，填充剂和聚二恶烷酮线的联合治疗是可能的。此外,发现聚二恶烷酮线似乎不会干扰SC-HA填料的交联反应，如果与较高pH的聚二恶烷酮一起使用，它可以增强交联反应并获得更高的粘度值。最后,这项研究得出了将混凝土用作SC-HA填料和聚二恶烷酮螺纹的增强棒的想法。
方法：使用6只小型猪（各3只雄性和3只雌性）进行了12周的实验，以评估SC-HA填充剂的作用。SC-HA填料的分子量固定在200kDa,并且检测G80、G250和G500的替代储能模量。该程序包括将SC-HA填充剂和聚二恶烷酮线注射到麻醉的小型猪的皮肤组织中，和组织取样后1个月（三只小型猪），和3个月(三只小型猪)进行组织学染色和分析。实验过程中观察到免疫反应。
结果：从业者报告说，将SC-HA填充剂与聚二恶烷酮线结合注射很容易。SC-HA填料的所有四种储能模量均可在含有聚二恶烷酮螺纹的套管内注射。此外，在手术过程中,在治疗部位没有免疫反应.组织学组织检查结果证实，SC-HA填料与现有聚二恶烷酮线之间没有化学相互作用，并且观察到SC-HA填料更均匀地分布在具有较低储能模量的组织内，导致周围填充物中胶原蛋白的产量更高。当与支架聚二恶烷酮线结合时，支架聚二恶烷酮线帮助填料均匀铺开，导致填充剂周围更均匀分布的胶原蛋白。
结论：今天，由于常规填充剂的高粘度，在一个程序中填充剂和聚二恶烷酮线的联合治疗具有挑战性。然而,这项研究证实，使用SC-HA填充剂，填充剂和聚二恶烷酮线的联合治疗是可能的。此外,发现聚二恶烷酮线似乎不会干扰SC-HA填料的交联反应，如果与较高pH的聚二恶烷酮一起使用，它可以增强交联反应并获得更高的粘度值。最后,这项研究得出了将混凝土用作SC-HA填料和聚二恶烷酮螺纹的增强棒的想法。
