PDF(Pubmed)
Abstract：
BACKGROUND: Soil microorganisms play an extensive role in the biogeochemical cycles providing the nutrients necessary for plant growth. Root-associated bacteria and fungi, originated from soil, are also known to influence host health. In response to environmental stresses, the plant roots exude specific molecules influencing the composition and functioning of the rhizospheric and root microbiomes. This response is host genotype-dependent and is affected by the soil microbiological and chemical properties. It is essential to unravel the influence of grapevine rootstock and scion genotypes on the composition of this microbiome, and to investigate this relationship with plant growth and adaptation to its environment. Here, the composition and the predicted functions of the microbiome of the root system were studied using metabarcoding on ten grapevine scion-rootstock combinations, in addition to plant growth and nutrition measurements.
RESULTS: The rootstock genotype significantly influenced the diversity and the structure of the bacterial and fungal microbiome, as well as its predicted functioning in rhizosphere and root compartments when grafted with the same scion cultivar. Based on β-diversity analyses, 1103P rootstock showed distinct bacterial and fungal communities compared to the five others (RGM, SO4, 41B, 3309 C and Nemadex). The influence of the scion genotype was more variable depending on the community and the investigated compartment. Its contribution was primarily observed on the β-diversity measured for bacteria and fungi in both root system compartments, as well as for the arbuscular mycorrhizal fungi (AMF) in the rhizosphere. Significant correlations were established between microbial variables and the plant phenotype, as well as with the plant mineral status measured in the petioles and the roots.
CONCLUSIONS: These results shed light on the capacity of grapevine rootstock and scion genotypes to recruit different functional communities of microorganisms, which affect host growth and adaptation to the environment. Selecting rootstocks capable of associating with positive symbiotic microorganisms is an adaptation tool that can facilitate the move towards sustainable viticulture and help cope with environmental constraints.
RESULTS: The rootstock genotype significantly influenced the diversity and the structure of the bacterial and fungal microbiome, as well as its predicted functioning in rhizosphere and root compartments when grafted with the same scion cultivar. Based on β-diversity analyses, 1103P rootstock showed distinct bacterial and fungal communities compared to the five others (RGM, SO4, 41B, 3309 C and Nemadex). The influence of the scion genotype was more variable depending on the community and the investigated compartment. Its contribution was primarily observed on the β-diversity measured for bacteria and fungi in both root system compartments, as well as for the arbuscular mycorrhizal fungi (AMF) in the rhizosphere. Significant correlations were established between microbial variables and the plant phenotype, as well as with the plant mineral status measured in the petioles and the roots.
CONCLUSIONS: These results shed light on the capacity of grapevine rootstock and scion genotypes to recruit different functional communities of microorganisms, which affect host growth and adaptation to the environment. Selecting rootstocks capable of associating with positive symbiotic microorganisms is an adaptation tool that can facilitate the move towards sustainable viticulture and help cope with environmental constraints.
摘要：
背景：土壤微生物在提供植物生长所必需的养分的生物地球化学循环中起着广泛的作用。与根相关的细菌和真菌，起源于土壤，也已知影响宿主健康。为了应对环境压力，植物根部渗出特定的分子，影响根际和根微生物组的组成和功能。这种反应是宿主基因型依赖性的，并且受土壤微生物和化学性质的影响。阐明葡萄砧木和接穗基因型对该微生物组组成的影响至关重要，并研究这种与植物生长和对环境的适应的关系。这里,在10种葡萄接穗砧木组合上，使用metabarcoding研究了根系微生物组的组成和预测功能，除了植物生长和营养测量。
结果：砧木基因型显著影响细菌和真菌微生物组的多样性和结构，以及用相同的接穗品种嫁接时在根际和根室中的预测功能。基于β多样性分析，与其他五种相比，1103P砧木显示出不同的细菌和真菌群落(RGM，SO4,41B,3309C和Nemadex）。接穗基因型的影响因群落和所研究的区室而异。它的贡献主要是在两个根系区室中测得的细菌和真菌的β多样性上观察到的，以及根际中丛枝菌根真菌（AMF）。在微生物变量和植物表型之间建立了显着的相关性，以及在叶柄和根部测量的植物矿物质状态。
结论：这些结果揭示了葡萄砧木和接穗基因型招募不同功能微生物群落的能力，影响寄主生长和对环境的适应。选择能够与积极共生微生物相关联的砧木是一种适应工具，可以促进迈向可持续葡萄栽培并帮助应对环境限制。
结果：砧木基因型显著影响细菌和真菌微生物组的多样性和结构，以及用相同的接穗品种嫁接时在根际和根室中的预测功能。基于β多样性分析，与其他五种相比，1103P砧木显示出不同的细菌和真菌群落(RGM，SO4,41B,3309C和Nemadex）。接穗基因型的影响因群落和所研究的区室而异。它的贡献主要是在两个根系区室中测得的细菌和真菌的β多样性上观察到的，以及根际中丛枝菌根真菌（AMF）。在微生物变量和植物表型之间建立了显着的相关性，以及在叶柄和根部测量的植物矿物质状态。
结论：这些结果揭示了葡萄砧木和接穗基因型招募不同功能微生物群落的能力，影响寄主生长和对环境的适应。选择能够与积极共生微生物相关联的砧木是一种适应工具，可以促进迈向可持续葡萄栽培并帮助应对环境限制。
