PDF(Pubmed)
Abstract：
In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19).
We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation.
Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower.
The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia.
We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation.
Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower.
The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia.
摘要：
背景：在这项研究中，对2019年严重冠状病毒病(COVID-19)继发的急性低氧性呼吸衰竭(AHRF)患者的支气管上皮内衬液(ELF)和血浆中炎性细胞因子的浓度进行了测定.
方法：我们综合分析了27例COVID-19AHRF患者的ELF和血浆中25种细胞因子的浓度。在对患者进行机械通气插管后，使用支气管微量采样方法通过气管内导管收集ELF。
结果：与健康志愿者相比，白细胞介素（IL）-6的浓度（中位数27.6pmol/L），IL-8（1045.1pmol/L），IL-17A（0.8pmol/L），IL-25（1.5pmol/L），COVID-19患者的ELF和IL-31（42.3pmol/L）明显高于志愿者。COVID-19患者血浆中MCP-1和MIP-1β的浓度明显高于志愿者。IL-8的ELF/血浆比例在25种细胞因子中最高，中位数为737，IL-6的ELF/血浆比率（中位数：218），IL-1β（202），IL-31(169),MCP-1(81),MIP-1β（55），TNF-α(47)较低。
结论：IL-6，IL-8，IL-17A的ELF浓度，IL-25和IL-31在COVID-19患者中显著升高。尽管在与ELF样本同时采集的血液样本中也检测到高水平的MIP-1和MIP-1β，结果表明肺部炎症是高度分隔的。我们的研究表明，对ELF中的细胞因子进行综合分析是了解重症肺炎患者肺部炎症和全身相互作用的可行方法。
方法：我们综合分析了27例COVID-19AHRF患者的ELF和血浆中25种细胞因子的浓度。在对患者进行机械通气插管后，使用支气管微量采样方法通过气管内导管收集ELF。
结果：与健康志愿者相比，白细胞介素（IL）-6的浓度（中位数27.6pmol/L），IL-8（1045.1pmol/L），IL-17A（0.8pmol/L），IL-25（1.5pmol/L），COVID-19患者的ELF和IL-31（42.3pmol/L）明显高于志愿者。COVID-19患者血浆中MCP-1和MIP-1β的浓度明显高于志愿者。IL-8的ELF/血浆比例在25种细胞因子中最高，中位数为737，IL-6的ELF/血浆比率（中位数：218），IL-1β（202），IL-31(169),MCP-1(81),MIP-1β（55），TNF-α(47)较低。
结论：IL-6，IL-8，IL-17A的ELF浓度，IL-25和IL-31在COVID-19患者中显著升高。尽管在与ELF样本同时采集的血液样本中也检测到高水平的MIP-1和MIP-1β，结果表明肺部炎症是高度分隔的。我们的研究表明，对ELF中的细胞因子进行综合分析是了解重症肺炎患者肺部炎症和全身相互作用的可行方法。
