Abstract：
Cervical carcinoma persists as a major global public health burden. While conventional therapeutic modalities inevitably cause ablation of adjacent non-tumorous tissues, photodynamic therapy (PDT) offers a targeted cytotoxic strategy through a photosensitizing agent (PS). However, the hydrophobicity and lack of selective accumulation of promising PS compounds such as zinc(II) phthalocyanine (ZnPc) impedes their clinical translation as standalone agents. The present study sought to incorporate ZnPc within double-layer hollow mesoporous silica nanoparticles (DHMSN) as nanocarriers to enhance aqueous dispersibility and tumor specificity. Owing to their compartmentalized design, the hollow mesoporous silica nanoparticles (HMSN) demonstrated enhanced ultrasonic imaging contrast. Combined with the vaporization of the perfluorocarbon perfluoropentane (PFP), the HMSN-encapsulated ZnPc enabled real-time ultrasound monitoring of PDT treatment.In vivo, the innate thermal energy induced vaporization of the DHMSN-carried PFP to significantly amplify ultrasound signals from the tumor site. Results demonstrated biocompatibility, efficient PFP microbubble generation, and robust photocatalytic activity. Collectively, this investigation establishes ultrasound-guided PDT utilizing multi-layer HMSN as a targeted therapeutic strategy for cervical malignancies with mitigated toxicity.
摘要：
宫颈癌仍然是全球主要的公共卫生负担。虽然传统的治疗方式不可避免地导致邻近的非肿瘤组织消融，光动力疗法（PDT）通过光敏剂（PS）提供了靶向的细胞毒性策略。然而,有前景的PS化合物如酞菁锌(II)(ZnPc)的疏水性和缺乏选择性积累阻碍了它们作为独立试剂的临床翻译。本研究试图将ZnPc掺入双层中空介孔二氧化硅纳米颗粒（DHMSN）中作为纳米载体，以增强水分散性和肿瘤特异性。由于它们的分隔设计，中空介孔二氧化硅纳米颗粒（HMSN）表现出增强的超声成像对比度。结合全氟化碳全氟戊烷（PFP）的汽化，HMSN封装的ZnPc能够对PDT治疗进行实时超声监测.在体内,先天性热能诱导DHMSN携带的PFP汽化，以显著放大来自肿瘤部位的超声信号。结果证明了生物相容性，高效的PFP微泡生成，和强大的光催化活性。总的来说，这项研究确立了利用多层HMSN作为宫颈恶性肿瘤靶向治疗策略的超声引导PDT,毒性减轻.
