Abstract:
Sex chromosomes have evolved in many plant species with separate sexes. Current plant research is shifting from examining the structure of sex chromosomes to exploring their functional aspects. New studies are progressively unveiling the specific genetic and epigenetic mechanisms responsible for shaping distinct sexes in plants. While the fundamental methods of molecular biology and genomics are generally employed for the analysis of sex chromosomes, it is often necessary to modify classical procedures not only to simplify and expedite analyses but sometimes to make them possible at all. In this review, we demonstrate how, at the level of structural and functional genetics, cytogenetics, and bioinformatics, it is essential to adapt established procedures for sex chromosome analysis.
摘要:
性染色体在许多具有不同性别的植物物种中已经进化。当前的植物研究正在从检查性染色体的结构转向探索其功能方面。新的研究正在逐步揭示负责塑造植物中不同性别的特定遗传和表观遗传机制。虽然分子生物学和基因组学的基本方法通常用于性染色体的分析,通常需要修改经典程序,不仅是为了简化和加快分析,有时也是为了使它们成为可能。在这次审查中,我们演示了如何,在结构和功能遗传学层面,细胞遗传学,和生物信息学,必须调整已建立的性染色体分析程序。